ArticleReproductive biology and endocrinology : RB&E2018
Three-dimensional evaluation of murine ovarian follicles using a modified CUBIC tissue clearing method.
Article in Reproductive biology and endocrinology : RB&E, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 22 citations in OpenAlex.
- A skeletal muscle atlas shows neuromuscular junction adaptations to growth and atrophy.Developmental cell · 2026Article
- Three-dimensional visualisation of human ovarian follicles using a whole-mount immunolabelling and optical tissue clearing method.Reproduction & fertility · 2025Article
- Treatment with trypLE before freezing improves thawing integrity and functionality of sheep ovarian tissue.Journal of ovarian research · 2025Article
- 3D visualization of uterus and ovary: tissue clearing techniques and biomedical applications.Frontiers in bioengineering and biotechnology · 2025Review
- Follicle-intrinsic and spatially distinct molecular programs drive follicle rupture and luteinization during ex vivo mammalian ovulation.Communications biology · 2024Article
- Deleterious variants in RNF111 impair female fertility and induce premature ovarian insufficiency in humans and mice.Science China. Life sciences · 2024Article
- Tissue clearing and imaging approaches for in toto analysis of the reproductive system†.Biology of reproduction · 2024Review
- Chronic stress induces meiotic arrest failure and ovarian reserve decline via the cAMP signaling pathway.Frontiers in endocrinology · 2023Article
- The role of TNFTNFα/p53 pathway in endometrial cancer mouse model administered with apple seed extract.Histology and histopathology · 2022Article
- Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse.Journal of visualized experiments : JoVE · 2021Article
- Time-Restricted Feeding Regulates Circadian Rhythm of Murine Uterine Clock.Current developments in nutrition · 2021Article
- Ovary Development: Insights From a Three-Dimensional Imaging Revolution.Frontiers in cell and developmental biology · 2021Review
- Comparison of methods for quantifying primordial follicles in the mouse ovary.Journal of ovarian research · 2020Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
- A novel third mesh-like myometrial layer connects the longitudinal and circular muscle fibers -A potential stratum to coordinate uterine contractions.Scientific reports · 2020Article
- Visualization of the Retina in Intact Eyes of Mice and Ferrets Using a Tissue Clearing Method.Translational vision science & technology · 2020Article
- Three-Dimensional Micro-Computed Tomography of the Adult Mouse Ovary.Frontiers in cell and developmental biology · 2020Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecently, we demonstrated the three-dimensional (3D) localization of murine trophoblast giant cells in the pregnant uterus using a modified Clear Unobstructed Brain Imaging Cocktails and Computational analysis (CUBIC) tissue-clearing method and hybrid construct consisting of the cytomegalovirus enhancer fused to the chicken beta-actin promoter (CAG) conjugated enhanced green fluorescent protein (EGFP) transgenic mice. In this study, we applied this method to obtain a transparent whole-image of the ovary and observed the 3D localization of individual oocytes in the developing follicles.
methodsOvarian samples were obtained from EGFP transgenic mice and subjected to nuclear staining with propidium iodide (PI) and CUBIC treatment. The detection of double fluorescence signals (green and red) and subsequent reconstruction of 3D images of the whole ovary were performed by light-sheet microscopy and computer programs, respectively.
resultsThe ovary became transparent using the CUBIC method and each nucleus of the follicle component cells was uniformly fluoro-stained by PI perfusion. In contrast, EGFP signals were strong in oocytes, whereas those of surrounding granulosa cells were faint. These signal differences in EGFP expression among oocytes, granulosa cells, and theca-interstitial cells produce well-contrasted images of the growing follicles, providing clear information of the 3D localization of individual oocytes.
conclusionThese results indicate that this procedure is one of the effective approaches to analyze the 3D structure of follicles in the whole ovary.
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