Evidence map›Paper›PMID 30071077›Full record

ArticlePloS one2018

Acinetobacter spp. porin Omp33-36: Classification and transcriptional response to carbapenems and host cells.

Katarina Novović, Sanja Mihajlović, Miroslav Dinić, Milka Malešević, Marija Miljković, Milan Kojić, Branko Jovčić

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.9field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Colistin Resistance inAntibiotics (Basel, Switzerland) · 2023
    Review
  7. Article
  8. PromisingFrontiers in immunology · 2022
    Review
  9. Article
  10. Genomic Characteristics of Colistin-ResistantAntibiotics (Basel, Switzerland) · 2020
    Article
  11. Antimicrobial agents and chemotherapy · 2020
    Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Katarina NovovićInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
Sanja MihajlovićInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
Miroslav DinićInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
Milka MaleševićInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
Marija MiljkovićInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
Milan KojićInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.ORCID 0000-0003-0808-6358
Branko JovčićInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.ORCID 0000-0002-9500-3786
University of Belgrade · RS

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acinetobacter baumannii has been recognized as one of the most challeging pathogens in clinical settings worldwide. Outer membrane porins play a significant role in Acinetobacter antibiotic resistance and virulence. A. baumannii carbapenem resistance and virulence factor porin Omp33-36 was the subject of this study. We investigated the omp33-36 gene transcriptional response in the growth phase, its response to carbapenems, and the effect of contact with host cells. Additionally, the cytotoxic effect of A. baumannii towards keratinocytes was assessed, as well as correlation between omp33-36 gene transcription and cytotoxicity. Further, Acinetobacter spp. Omp33-36 was classified and its characteristics relevant for vaccine candidature were determined. The level of the omp33-36 gene transcription varied between growth phases, but a common pattern could not be established among different strains. Treatment with subinhibitory concentrations of carbapenems decreased, while contact with keratinocytes increased omp33-36 expression in the analysed A. baumannii strains. Variations in omp33-36 mRNA levels did not correlate with cytotoxicity levels. Decrease of omp33-36 mRNA during treatment with subinhibitory concentrations of carbapenems, indicated the importance of transcriptional changes in reversible resistance to carbapenems due to the absence of Omp33-36. The transcription of omp33-36 increased after contact with keratinocytes, indicating the important role of de novo transcription during the initial phase of A. baumannii infection. Primary structural analysis of Acinetobacter spp. Omp33-36 revealed three distinct groups (among four A. baumannii variants). Although we have shown that Omp33-36 was highly polymorphic, we propose a potential antigen (PLAEAAFL motif) for vaccine development. According to PROVEAN analysis, the highly polymorphic structure of Omp33-36 porin should not influence its function significantly.

Indexed as

Acinetobacter baumanniiCarbapenemsCell LineDrug Resistance, BacterialHumansKeratinocytesPhylogenyRNA, MessengerTranscription, GeneticVirulence FactorsCarbapenemsRNA, MessengerVirulence Factors

Identifiers

PMID30071077
PMCPMC6072067
OpenAlexW2885062401

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.