ArticlePLoS neglected tropical diseases2018
Infection of epididymal epithelial cells and leukocytes drives seminal shedding of Zika virus in a mouse model.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Crimean-Congo hemorrhagic fever virus localization and shedding in the reproductive tract of lethal and survivor mouse models.Emerging microbes & infections · 2026Article
- Neonatal Fc receptor is a functional receptor for classical human astrovirus.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- CD8Npj viruses · 2024Article
- Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon.Journal of virology · 2023Article
- CLEC5A mediates Zika virus-induced testicular damage.Journal of biomedical science · 2023Article
- Mouse models of Ebola virus tolerance and lethality: characterization of CD-1 mice infected with wild-type, guinea pig-adapted, or mouse-adapted virus.Antiviral research · 2023Article
- Review
- Early infection of Zika virus in the male reproductive system of AG129 mice: molecular and immunohistochemical evaluation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Zika virus persistence in the male macaque reproductive tract.PLoS neglected tropical diseases · 2022Article
- Human Neutrophils Present Mild Activation by Zika Virus But Reduce the Infection of Susceptible Cells.Frontiers in immunology · 2022Article
- Flow-FISH as a Tool for Studying Bacteria, Fungi and Viruses.Biotech (Basel (Switzerland)) · 2021Review
- Persistence of Zika virus RNA in the epididymis of the murine male reproductive tract.Virology · 2021Article
- The Specificity of the Persistent IgM Neutralizing Antibody Response in Zika Virus Infections among Individuals with Prior Dengue Virus Exposure.Journal of clinical microbiology · 2021Article
- Epididymal epithelium propels early sexual transmission of Zika virus in the absence of interferon signaling.Nature communications · 2021Article
- Zika virus disrupts the barrier structure and Absorption/Secretion functions of the epididymis in mice.PLoS neglected tropical diseases · 2021Article
- Zika virus in rhesus macaque semen and reproductive tract tissues: a pilot study of acute infection†.Biology of reproduction · 2020Article
- Zika Virus Replication in Myeloid Cells during Acute Infection Is Vital to Viral Dissemination and Pathogenesis in a Mouse Model.Journal of virology · 2020Article
- The Cellular Impact of the ZIKA Virus on Male Reproductive Tract Immunology and Physiology.Cells · 2020Review
- Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.Viruses · 2020Article
- Duration of seminal Zika viral RNA shedding in immunocompetent mice inoculated with Asian and African genotype viruses.Virology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While primarily a mosquito-borne virus, Zika virus (ZIKV; genus Flavivirus in the Flaviviridae family) is capable of being sexually transmitted. Thirty to fifty percent of men with confirmed ZIKV infection shed ZIKV RNA in their semen, and prolonged viral RNA shedding in semen can occur for more than 6 months. The cellular reservoir of ZIKV in semen is unknown, although spermatozoa have been shown to contain ZIKV RNA and antigen. Yet, spermatozoa are not a requisite for sexual transmission, as at least one case of ZIKV sexual transmission involved a vasectomized man. To determine the cellular reservoirs of ZIKV in semen, an established animal model of sexual transmission was used. The majority of virus detected in the seminal fluid of infected mice during the peak timing of sexual transmission was from the supernatant fraction, suggesting cell-free ZIKV may be largely responsible for sexual transmission. However, some ZIKV RNA was cell-associated. In the testes and epididymides of infected mice, intracellular staining of ZIKV RNA was more pronounced in spermatogenic precursors (spermatocytes and spermatogonia) than in spermatids. Visualization of intracellular negative strand ZIKV RNA demonstrated ZIKV replication intermediates in leukocytes, immature spermatids and epididymal epithelial cells in the male urogenital tract. Epididymal epithelial cells were the principal source of negative-strand ZIKV RNA during the peak timing of sexual transmission potential, indicating these cells may be the predominant source of infectious cell-free ZIKV in seminal fluid. These data promote a more complete understanding of sexual transmission of ZIKV and will inform further model development for future studies on persistent ZIKV RNA shedding.
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