Evidence map›Paper›PMID 30070754›Full record

ArticleChembiochem : a European journal of chemical biology2018

Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy.

Manabendra Das, Yang Du, Jonas S Mortensen, Parameswaran Hariharan, Hyun Sung Lee, Bernadette Byrne, Claus J Loland, Lan Guan, Brian K Kobilka, Pil Seok Chae

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 5 countries.

Manabendra DasDepartment of Bionanotechnology, Hanyang University, 55 Hanyangdaehak-ro, Ansan, 155-88, Korea.ORCID 0000-0003-3690-3183
Yang DuMolecular and Cellular Physiology, Stanford University, 279 Campus Drive, Stanford, CA, 94305, USA.ORCID 0000-0001-5115-0736
Jonas S MortensenDepartment of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200, Copenhagen, Denmark.ORCID 0000-0002-6743-276X
Parameswaran HariharanDepartment of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, 3601 4th St. MS 6551, Lubbock, TX, 79430, USA.ORCID 0000-0002-6020-1547
Hyun Sung LeeDepartment of Bionanotechnology, Hanyang University, 55 Hanyangdaehak-ro, Ansan, 155-88, Korea.ORCID 0000-0002-2465-6528
Bernadette ByrneDepartment of Life Sciences, Imperial College London, Exhibition Road, London, SW7 2AZ, UK.ORCID 0000-0001-9598-9832
Claus J LolandDepartment of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200, Copenhagen, Denmark.ORCID 0000-0002-1773-1446
Lan GuanDepartment of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, 3601 4th St. MS 6551, Lubbock, TX, 79430, USA.ORCID 0000-0002-2274-361X
Brian K KobilkaMolecular and Cellular Physiology, Stanford University, 279 Campus Drive, Stanford, CA, 94305, USA.ORCID 0000-0001-5958-3990
Pil Seok ChaeDepartment of Bionanotechnology, Hanyang University, 55 Hanyangdaehak-ro, Ansan, 155-88, Korea.ORCID 0000-0003-1799-3304
Hanyang University · KRStanford University · USTexas Tech University · USUniversity of Copenhagen · DKImperial College London · GBUniversity of Kaiserslautern · DE

Funding

Integrated approaches to symport mechanisms of membrane transportersR01GM122759 · NIGMS · TEXAS TECH UNIVERSITY HEALTH SCIS CENTER · PI GUAN, LAN · 2018 to 2021
$1.4M
Recombinant expression and functional characterization of a lysophosphatidylcholine transporterR21NS105863 · NINDS · TEXAS TECH UNIVERSITY HEALTH SCIS CENTER · PI GUAN, LAN · 2018 to 2019
$421k
NIGMS NIH HHS R01 GM122759NINDS NIH HHS R21 NS105863
6 · The paper itself

Abstract

A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750-16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.

Indexed as

Amino Acid Transport SystemsBacterial ProteinsDetergentsHumansHydrophobic and Hydrophilic InteractionsMembrane ProteinsMicellesProtein StabilityReceptors, Adrenergic, beta-2Salmonella typhimuriumSolubilitySymportersAmino Acid Transport SystemsBacterial ProteinsDetergentsmelibiose permeaseMembrane ProteinsMicellesReceptors, Adrenergic, beta-2Symportersamphiphilesdetergent designdetergent facialityprotein stabilizationprotein structures

Identifiers

PMID30070754
PMCPMC7233144
OpenAlexW2885307184

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.