Evidence map›Paper›PMID 30070085›Full record

ArticleJournal of Zhejiang University. Science. B

High-resolution melting-based TILLING of γ ray-induced mutations in rice.

Shan Li, Song-Mei Liu, Hao-Wei Fu, Jian-Zhong Huang, Qing-Yao Shu

Open access · greenAbstract read
In one paragraph

Article in Journal of Zhejiang University. Science. B. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Review
  5. Is it the end of TILLING era in plant science?Frontiers in plant science · 2023
    Review
  6. Article
  7. Breeding crops by design for future agriculture.Journal of Zhejiang University. Science. B · 2020
    Article
  8. Breeding for low cadmium accumulation cereals.Journal of Zhejiang University. Science. B · 2020
    Review
  9. Article
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Shan LiNational Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, China.
Song-Mei LiuNational Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, China.
Hao-Wei FuJiaxing Academy of Agricultural Sciences, Jiaxing 314016, China.
Jian-Zhong HuangInstitute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
Qing-Yao ShuNational Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, China.
Institute of Crop Sciences · CNZhejiang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics strategy for the high-throughput screening of induced mutations. γ radiation, which often induces both insertion/deletion (Indel) and point mutations, has been widely used in mutation induction and crop breeding. The present study aimed to develop a simple, high-throughput TILLING system for screening γ ray-induced mutations using high-resolution melting (HRM) analysis. Pooled rice (Oryza sativa) samples mixed at a 1:7 ratio of Indel mutant to wild-type DNA could be distinguished from the wild-type controls by HRM analysis. Thus, an HRM-TILLING system that analyzes pooled samples of four M

Indexed as

INDEL MutationMutagenesisCrops, AgriculturalGamma RaysGenetic TechniquesGenome, PlantHomozygoteOryzaPlant BreedingPolymerase Chain ReactionSeedsSequence Analysis, DNASequence DeletionMutation screening; High-resolution melting (HRM) analysis; Targeting Induced Local Lesions IN Genomes (TILLING); Mutant; Indel; γ ray; Rice

Identifiers

PMID30070085
PMCPMC6102187
OpenAlexW2887514435

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.