ArticleNature communications2018
Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
What it found
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Who cites it
97 citing papers in PubMed, 236 citations in OpenAlex.
- Infectious transgenesis in arthropods.bioRxiv : the preprint server for biology · 2026Article
- A novel needleless delivery of Cas9 ribonucleoprotein complex in Bemisia tabaci embryos for gene editing.World journal of microbiology & biotechnology · 2026Article
- CRISPR-Cas9 mediated knockout of the white gene in the bluetongue virus vector, Culicoides sonorensis (biting midge).Scientific reports · 2026Article
- Article
- DsiRNA-mediated silencing of Ceratitis capitata transformer or transformer-2 leads to masculinization of XX embryos and systemic gene silencing in ovaries.Insect science · 2026Article
- HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects.Communications biology · 2026Article
- SYNCAS-mediated CRISPR-Cas9 genome editing in the Jewel wasp, Nasonia vitripennis.Insect molecular biology · 2026Article
- Snail 3G: genomics, genetics, and gene-editing ofFrontiers in genetics · 2026Review
- Sindbis virus is suppressed in the yellow fever mosquitoAutophagy · 2025Article
- Pheromone-Binding Proteins in Pest Control: From Molecular Insights to Real-World Applications.Journal of agricultural and food chemistry · 2025Review
- Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.Frontiers in zoology · 2025Review
- CRISPR technologies for the control and study of malaria-transmitting anopheline mosquitoes.Parasites & vectors · 2025Review
- Review
- Carrier-free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapy.Fundamental research · 2025Article
- CRISPR/Cas9-Mediated Knockout of the White Gene inInternational journal of molecular sciences · 2025Article
- Article
- Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9.Journal of visualized experiments : JoVE · 2025Article
- CRISPR/Cas9 Genome Editing in the Diamondback Moth: Current Progress, Challenges, and Prospects.International journal of molecular sciences · 2025Review
- SYNCAS based CRISPR-Cas9 gene editing in predatory mites, whiteflies and stinkbugs.Insect biochemistry and molecular biology · 2025Article
- Adapting and Testing ReMOT Control for Expanded CRISPR-Era Genome Functions in Non-model Insects.Methods in molecular biology (Clifton, N.J.) · 2025Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Cas9-mediated gene editing is a powerful tool for addressing research questions in arthropods. Current approaches rely upon delivering Cas9 ribonucleoprotein (RNP) complex by embryonic microinjection, which is challenging, is limited to a small number of species, and is inefficient even in optimized taxa. Here we develop a technology termed Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) to deliver Cas9 RNP to the arthropod germline by injection into adult female mosquitoes. We identify a peptide (P2C) that mediates transduction of Cas9 RNP from the female hemolymph to the developing mosquito oocytes, resulting in heritable gene editing of the offspring with efficiency as high as 0.3 mutants per injected mosquito. We demonstrate that P2C functions in six mosquito species. Identification of taxa-specific ovary-specific ligand-receptor pairs may further extend the use of ReMOT Control for gene editing in novel species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.