ArticleMolecular microbiology2018
Peptidoglycan degradation machinery in Clostridium difficile forespore engulfment.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Reactions of SleC, Its Structure and Inhibition in Mitigation of Spore Germination inJournal of the American Chemical Society · 2025Article
- Cleavage of an engulfment peptidoglycan hydrolase by a sporulation signature protease in Clostridioides difficile.Molecular microbiology · 2024Article
- Clostridioides difficile Sporulation.Advances in experimental medicine and biology · 2024Review
- Masters of Misdirection: Peptidoglycan Glycosidases in Bacterial Growth.Journal of bacteriology · 2023Review
- Conservation and Evolution of the Sporulation Gene Set in Diverse Members of theJournal of bacteriology · 2022Article
- Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction.PLoS pathogens · 2021Article
- Structural, Metabolic and Evolutionary Comparison of Bacterial Endospore and Exospore Formation.Frontiers in microbiology · 2021Review
- Pharmacokinetics of CamSA, a potential prophylactic compound against Clostridioides difficile infections.Biochemical pharmacology · 2021Article
- Distribution and preservation of the components of the engulfment. What is beyond representative genomes?PloS one · 2021Article
- Milestones inMicrobial cell (Graz, Austria) · 2020Review
- Role of SpoIVA ATPase Motifs during Clostridioides difficile Sporulation.Journal of bacteriology · 2020Article
- Shaping an Endospore: Architectural Transformations DuringAnnual review of microbiology · 2020Review
- From Root to Tips: Sporulation Evolution and Specialization in Bacillus subtilis and the Intestinal Pathogen Clostridioides difficile.Molecular biology and evolution · 2019Article
- Article
- The Transcriptional Regulator Lrp Contributes to Toxin Expression, Sporulation, and Swimming Motility inFrontiers in cellular and infection microbiology · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Clostridium difficile remains the leading cause of antibiotic-associated diarrhoea in hospitals worldwide, linked to significant morbidity and mortality. As a strict anaerobe, it produces dormant cell forms - spores - which allow it to survive in the aerobic environment. Importantly, spores are the transmission agent of C. difficile infections. A key aspect of sporulation is the engulfment of the future spore by the mother cell and several proteins have been proposed to be involved. Here, we investigated the role of the SpoIID, SpoIIM and SpoIIP (DMP) machinery and its interplay with the SpoIIQ:SpoIIIAH (Q:AH) complex in C. difficile. We show that, surprisingly, SpoIIM, the proposed machinery anchor, is not required for efficient engulfment and sporulation. We demonstrate the requirement of DP for engulfment due to their sequential peptidoglycan degradation activity, both in vitro and in vivo. Finally, new interactions within DMP and between DMP and Q:AH suggest that both systems form a single engulfment machinery to keep the mother cell and forespore membranes together throughout engulfment. This work sheds new light upon the engulfment process and on how different sporeformers might use the same components in different ways to drive spore formation.
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