ArticleFrontiers in physiology2018
ATP Content and Cell Viability as Indicators for Cryostress Across the Diversity of Life.
Article in Frontiers in physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
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- Bioluminescent test systems based on firefly luciferase for studying stress effects on living cells.Biophysical reviews · 2022Review
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- Altitudinal Variation of Metabolites, Mineral Elements and Antioxidant Activities ofMolecules (Basel, Switzerland) · 2021Article
- Oxidative and salt stresses alter the 26S proteasome holoenzyme and associated protein profiles in Arabidopsis thaliana.BMC plant biology · 2021Article
- Subcellular Proteomics to Understand Promotive Effect of Plant-Derived Smoke Solution on Soybean Root.Proteomes · 2021Article
- Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?Applied microbiology and biotechnology · 2021Review
- Measuring and modeling energy and power consumption in living microbial cells with a synthetic ATP reporter.BMC biology · 2021Article
- Systemic delivery of a specific antibody targeting the pathological N-terminal truncated tau peptide reduces retinal degeneration in a mouse model of Alzheimer's Disease.Acta neuropathologica communications · 2021Article
- Changes in the Concentration of Purine and Pyridine as a Response to Single Whole-Body Cryostimulation.Frontiers in physiology · 2021Article
- Hibernator-Derived Cells Show Superior Protection and Survival in Hypothermia Compared to Non-Hibernator Cells.International journal of molecular sciences · 2020Article
- Factors determining microbial colonization of liquid nitrogen storage tanks used for archiving biological samples.Applied microbiology and biotechnology · 2020Article
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- Assessing the viability of genebanked seeds from rare, wild plants native to the United States using the D.E.A.D. paradigm.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many natural environments, organisms get exposed to low temperature and/or to strong temperature shifts. Also, standard preservation protocols for live cells or tissues involve ultradeep freezing in or above liquid nitrogen (-196°C or -150°C, respectively). To which extent these conditions cause cold- or cryostress has rarely been investigated systematically. Using ATP content as an indicator of the physiological state of cells, we found that representatives of bacteria, fungi, algae, plant tissue, as well as plant and human cell lines exhibited similar responses during freezing and thawing. Compared to optimum growth conditions, the cellular ATP content of most model organisms decreased significantly upon treatment with cryoprotectant and cooling to up to -196°C. After thawing and a longer period of regeneration, the initial ATP content was restored or even exceeded the initial ATP levels. To assess the implications of cellular ATP concentration for the physiology of cryostress, cell viability was determined in parallel using independent approaches. A significantly positive correlation of ATP content and viability was detected only in the cryosensitive algae
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.