SynthesisMicrobial cell factories2018
Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review.
Synthesis in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
12 citing papers in PubMed.
- A protein-fragment complementation assay to quantify synthetic protein scaffold efficiency.Synthetic biology (Oxford, England) · 2026Article
- Surface display of eugenol oxidase and dioxygenase complex as a sustainable biocatalyst for efficient bioconversion of lignin-derived 4-n-propylguaiacol to vanillin.Microbial cell factories · 2025Article
- Harnessing organelle engineering to facilitate biofuels and biochemicals production in yeast.Journal of microbiology (Seoul, Korea) · 2025Review
- PvdL Orchestrates the Assembly of the Nonribosomal Peptide Synthetases Involved in Pyoverdine Biosynthesis inInternational journal of molecular sciences · 2024Article
- Exploring linker's sequence diversity to fuse carotene cyclase and hydroxylase for zeaxanthin biosynthesis.Metabolic engineering communications · 2023Article
- Self assembling nanoparticle enzyme clusters provide access to substrate channeling in multienzymatic cascades.Nature communications · 2023Article
- Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system.Synthetic and systems biotechnology · 2022Article
- Synthetic Scaffold Systems for Increasing the Efficiency of Metabolic Pathways in Microorganisms.Biology · 2021Review
- Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective.Frontiers in bioengineering and biotechnology · 2021Review
- Enzyme Assembly for Compartmentalized Metabolic Flux Control.Metabolites · 2020Review
- Engineering of Metabolic Pathways Using Synthetic Enzyme Complexes.Plant physiology · 2019Review
- Enzymatic Bioremediation of Organophosphate Compounds-Progress and Remaining Challenges.Frontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
For years, microbes have been widely applied as chassis in the construction of synthetic metabolic pathways. However, the lack of in vivo enzyme clustering of heterologous metabolic pathways in these organisms often results in low local concentrations of enzymes and substrates, leading to a low productive efficacy. In recent years, multiple methods have been applied to the construction of small metabolic clusters by spatial organization of heterologous metabolic enzymes. These methods mainly focused on using engineered molecules to bring the enzymes into close proximity via different interaction mechanisms among proteins and nucleotides and have been applied in various heterologous pathways with different degrees of success while facing numerous challenges. In this paper, we mainly reviewed some of those notable advances in designing and creating approaches to achieve spatial organization using different intermolecular interactions. Current challenges and future aspects in the further application of such approaches are also discussed in this paper.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.