ArticleBMC genomics2018
Effects of liquid cultivation on gene expression and phenotype of C. elegans.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
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- High-Throughput Toxicity Screening withEnvironmental science & technology · 2026Review
- Caenorhabditis elegans as an experimental model for resilience-boosting microbiota interventions in nonmodel ectotherms.FEMS microbiology reviews · 2026Review
- Comparative analysis of nanosilver toxicity in C. elegans: influence of exposure media on accumulation, physiological and biochemical effects.Environmental science and pollution research international · 2026Article
- De novo NADCommunications biology · 2025Article
- Intra-serotype variation ofMicrobiology spectrum · 2025Article
- GelDrop Array High-Throughput Screening in Nematodes.microPublication biology · 2025Article
- Use of Viscous medium to study anthelmintic drug action in Caenorhabditis elegans.Scientific reports · 2024Article
- Article
- Increasing Complexity of the N-Glycome During Caenorhabditis Development.Molecular & cellular proteomics : MCP · 2023Article
- Article
- Long-Term Culture and Monitoring of Isolated Caenorhabditis elegans on Solid Media in Multi-Well Devices.Journal of visualized experiments : JoVE · 2022Article
- Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions.Journal of visualized experiments : JoVE · 2022Article
- Axenic Culture ofInternational journal of molecular sciences · 2022Article
- Extracellular Vesicles From Microalgae: Uptake Studies in Human Cells andFrontiers in bioengineering and biotechnology · 2022Article
- Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations.Journal of visualized experiments : JoVE · 2021Article
- Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction ofSTAR protocols · 2021Article
- Phenotypic Screening inPharmaceuticals (Basel, Switzerland) · 2020Review
- Biology of theGenetics · 2019Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundLiquid cultures have been commonly used in space, toxicology, and pharmacology studies of Caenorhabditis elegans. However, the knowledge about transcriptomic alterations caused by liquid cultivation remains limited. Moreover, the impact of different genotypes in rapid adaptive responses to environmental changes (e.g., liquid cultivation) is often overlooked. Here, we report the transcriptomic and phenotypic responses of laboratory N2 and the wild-isolate AB1 strains after culturing P
resultsSignificant variations were found in the gene expressions between the N2 and AB1 strains in response to liquid cultivation. The results demonstrated that 8-34% of the environmental change-induced transcriptional responses are transmitted to the subsequent generation. By categorizing the gene expressions for genotype, environment, and genotype-environment interactions, we identified that the genotype has a substantial impact on the adaptive responses. Functional analysis of the transcriptome showed correlation with phenotypical changes. For example, the N2 strain exhibited alterations in both phenotype and gene expressions for germline and cuticle in axenic liquid cultivation. We found transcript evidence to approximately 21% of the computationally predicted genes in C. elegans by exposing the worms to environmental changes.
conclusionsThe presented study reveals substantial differences between N2 and AB1 strains for transcriptomic and phenotypical responses to rapid environmental changes. Our data can provide standard controls for future studies for the liquid cultivation of C. elegans and enable the discovery of condition-specific genes.
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