ArticleJournal of the American Chemical Society2018
Mimicking Co-Transcriptional RNA Folding Using a Superhelicase.
Article in Journal of the American Chemical Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Sequential structure probing of cotranscriptional RNA folding intermediates.Nature communications · 2025Article
- Article
- Observation of coordinated RNA folding events by systematic cotranscriptional RNA structure probing.Nature communications · 2023Article
- Mind your tag in single-molecule measurements.Cell reports methods · 2023Article
- Helicase Activity Modulation with On-Demand Light-Based Conformational Control.Journal of the American Chemical Society · 2023Article
- Linking folding dynamics and function of SAM/SAH riboswitches at the single molecule level.Nucleic acids research · 2023Article
- Emerging Quantitative Biochemical, Structural, and Biophysical Methods for Studying Ribosome and Protein-RNA Complex Assembly.Biomolecules · 2023Review
- Observation of structural switch in nascent SAM-VI riboswitch during transcription at single-nucleotide and single-molecule resolution.Nature communications · 2023Article
- Single Molecule FRET Analysis of CRISPR Cas9 Single Guide RNA Folding Dynamics.The journal of physical chemistry. B · 2023Article
- Dynamic Refolding of OxyS sRNA by the Hfq RNA Chaperone.Journal of molecular biology · 2022Article
- How does RNA fold dynamically?Journal of molecular biology · 2022Review
- DNA-RNA hybrid G-quadruplex tends to form near the 3' end of telomere overhang.Biophysical journal · 2022Article
- Peptides before and during the nucleotide world: an origins story emphasizing cooperation between proteins and nucleic acids.Journal of the Royal Society, Interface · 2022Review
- An anionic ligand snap-locks a long-range interaction in a magnesium-folded riboswitch.Nature communications · 2022Article
- Optical tweezer and TIRF microscopy for single molecule manipulation of RNA/DNA nanostructures including their rubbery property and single molecule counting.Biophysics reports · 2021Article
- Monitoring RNA dynamics in native transcriptional complexes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A roadmap for rRNA folding and assembly during transcription.Trends in biochemical sciences · 2021Review
- Transcription complexes as RNA chaperones.Transcription · 2021Article
- Transcription Regulation Through Nascent RNA Folding.Journal of molecular biology · 2021Review
- Genome oligopaint via local denaturation fluorescence in situ hybridization.Molecular cell · 2021Article
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Authors and funding
5 authors.
Funding
Abstract
Vectorial folding of RNA during transcription can produce intermediates with distinct biochemical activities. Here, we design an artificial minimal system to mimic cotranscriptional RNA folding in vitro. In this system, a presynthesized RNA molecule begins to fold from its 5'-end, as it is released from a heteroduplex by an engineered helicase that translocates on the complementary DNA strand in the 3'-to-5' direction. This chemically stabilized "superhelicase" Rep-X processively unwinds thousands of base pairs of DNA. The presynthesized RNA enables us to flexibly position fluorescent labels on the RNA for single-molecule fluorescence resonance energy transfer analysis and allows us to study real-time conformational dynamics during the vectorial folding process. We observed distinct signatures of the maiden secondary and tertiary folding of the Oryza sativa twister ribozyme. The maiden vectorial tertiary folding transitions occurred faster than Mg
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.