ArticleArchives of virology2018
Preparation and characterization of an attenuated porcine epidemic diarrhea virus strain by serial passaging.
Article in Archives of virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.Discover nano · 2025Review
- Serial passage of PDCoV in cell culture reduces its pathogenicity and its damage of gut microbiota homeostasis in piglets.mSystems · 2024Article
- Article
- A porcine epidemic diarrhea virus strain with distinct characteristics of four amino acid insertion in the COE region of spike protein.Veterinary microbiology · 2021Article
- Article
- Regulatory Effect of Methylation of the PorcineGenes · 2020Article
- Attenuation and characterization of porcine enteric alphacoronavirus strain GDS04 via serial cell passage.Veterinary microbiology · 2019Article
- Characterization and pathogenicity of Vero cell-attenuated porcine epidemic diarrhea virus CT strain.Virology journal · 2019Article
- Engineering a Live Attenuated Porcine Epidemic Diarrhea Virus Vaccine Candidate via Inactivation of the Viral 2'-Journal of virology · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Porcine epidemic diarrhea virus (PEDV) is prevalent in most parts of the world. Owing to its antigenic variation, prevention of the diseases caused by this virus is difficult. In this study, two PEDV isolates with similar growth kinetics were successfully propagated in Vero cells. Complete genome sequence analysis showed that they have a 49nt deletion in the ORF3 gene and were classified into Group 1, the same group that includes the classical CV777 strain. Recombination analysis revealed that the event had occurred in the ORF1a gene, at 3596-6819 nt, among the two PEDV isolates and the CV777 and DR13 strains. During their continuous propagation, 14 nonsynonymous mutations occurred in the spike (S) gene of strain JS-2/2014 between generations G5 and G90, but there were no changes between G90 and G100. We assumed that strain JS-2/2014 might be attenuated by the 90th generation. Piglets orally fed with JS-2/2014 G90 showed no clinical symptoms, and no virus was detected in the feces and nasal fluid. In conclusion, JS-2/2014 was successfully identified by screening, was attenuated after propagation in Vero cells, and may serve as a candidate virus for vaccine preparations.
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Registered trials
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