Evidence map›Paper›PMID 30062424›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Using GAL4-Inducible Transgenics to Modulate Rho GTPase Activity in Zebrafish.

Nicholas J Hanovice, Emily McMains, Jeffrey M Gross

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Nicholas J HanoviceDepartments of Ophthalmology and Developmental Biology, Louis J Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Emily McMainsInstitute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
Jeffrey M GrossDepartments of Ophthalmology and Developmental Biology, Louis J Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. grossjm@pitt.edu.
University of Pittsburgh · USThe University of Texas at Austin · US

Funding

ZEBRAFISH INTERNATIONAL RESOURCE CENTERP40RR012546 · NCRR · UNIVERSITY OF OREGON · PI WESTERFIELD, MONTE · 1998 to 2011
$21.9M
Virus Production and Manipulation of Protein/Gene Expression ModuleP30EY008098 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Ethan A Rossi · 1989 to 2026
$17.8M
Genetic Regulation of Eye Development in ZebrafishR01EY018005 · NEI · UNIVERSITY OF TEXAS AT AUSTIN · PI GROSS, JEFFREY · 2008 to 2017
$3.3M
NCRR NIH HHS P40 RR012546NEI NIH HHS P30 EY008098NEI NIH HHS R01 EY018005
6 · The paper itself

Abstract

Rho GTPases are Ras-family G proteins that regulate many critical cellular functions. Due to their requirement during early embryonic development, investigations into the function of Rho GTPases at a tissue-specific level require inducible and spatially targeted modulation of Rho GTPase activity. Here, we describe the use of ten novel zebrafish transgenics enabling GAL4-specific expression of Rho GTPases to modulate Rho GTPase activity with spatial and temporal control.

Indexed as

Animals, Genetically ModifiedDNA-Binding ProteinsEmbryonic Developmentrho GTP-Binding ProteinsTranscription FactorsZebrafishZebrafish ProteinsAnimalsEmbryo, NonmammalianGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicDNA-Binding ProteinsGal4 protein, zebrafishrho GTP-Binding ProteinsTranscription FactorsZebrafish ProteinsCdc42GAL4/UASHeat shockRac1RhoATransgenicsZebrafish

Identifiers

PMID30062424
PMCPMC6891047
OpenAlexW2885198842

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.