ArticleCell research2018
Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis.
Article in Cell research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 236 papers, 1 of them a synthesis that pooled it.
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Who cites it
236 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Inflammation-mediated effects of diabetes mellitus on male fertility: a systematic review and meta-analysis.Frontiers in endocrinology · 2025Pooled it
- Epigenetic dynamics of human spermatogenesis and their dysregulation in non-obstructive azoospermia.Protein & cell · 2026Article
- DUET-seq: An Open-Source Droplet Platform for High-Fidelity Joint Chromatin and Transcriptome Profiling Reveals Temporal Regulatory Decoupling in Single Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Sertoli cell oxidative stress as a convergent hazard endpoint for environmental toxicants: A systematic review, meta-analysis, and AOP-based assessment.Redox biology · 2026Review
- Article
- The pachytene-specific lncRNANon-coding RNA research · 2026Article
- Cross-species comparative transcriptomic analysis of spermatozoa reveals species-specific regulatory networks linked to fertilization-associated genes in cattle, sheep and goats.Science China. Life sciences · 2026Article
- SYCP2 recruits HORMAD2 to chromosome axes for unsynapsed chromatin silencing and synapsis surveillance in meiosis.Nature communications · 2026Article
- The constitutive 20S proteasome is required for the maintenance and differentiation of spermatogonia in mice.Journal of advanced research · 2026Article
- Single cell analysis of developing Merkel cells reveals the emergence of non-coding RNA biotypes as a hallmark of terminal differentiation.Cell death and differentiation · 2026Article
- SOX30 Governs Synaptonemal Complex Assembly and Homologous Recombination in Male Meiosis.Cell proliferation · 2026Article
- DAXX directs dual modes of H3.4-to-H3.3 histone replacement in the male germline.Genes & development · 2026Article
- Digital Chromosome Banding Reveals Distinct Spatiotemporal Dynamics and Sexual Dimorphism in Meiotic Silencing.bioRxiv : the preprint server for biology · 2026Article
- LSM7 coordinates scaRNA-mediated snRNA modification to ensure spliceosome fidelity and spermatogonial stem cell differentiation.Cell death and differentiation · 2026Article
- Defective transcription of AAGAG satellite DNA causes sex-ratio meiotic drive in Drosophila.Nature communications · 2026Article
- The transcription factor E4F1 is crucial for spermatogonial differentiation and meiosis progression in mice.BMC genomics · 2026Article
- Defective transcription of AAGAG satellite DNA causes sex-ratio meiotic drive inbioRxiv : the preprint server for biology · 2026Article
- Reconstitution of spermatogenesis and continuous generation of functional haploid germ cells in mouse testicular organoids.Nature communications · 2026Article
- Nucleolar migration regulates meiotic sex chromosome inactivation via phase separation during mammalian spermatogenesis.Nature communications · 2026Article
- ATF4 Is Dispensable for Spermatogenesis but Protective Against ER Stress Under Normal Conditions.Biology · 2026Article
176 more citing papers are in PubMed but not listed here.
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15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A systematic interrogation of male germ cells is key to complete understanding of molecular mechanisms governing spermatogenesis and the development of new strategies for infertility therapies and male contraception. Here we develop an approach to purify all types of homogeneous spermatogenic cells by combining transgenic labeling and synchronization of the cycle of the seminiferous epithelium, and subsequent single-cell RNA-sequencing. We reveal extensive and previously uncharacterized dynamic processes and molecular signatures in gene expression, as well as specific patterns of alternative splicing, and novel regulators for specific stages of male germ cell development. Our transcriptomics analyses led us to discover discriminative markers for isolating round spermatids at specific stages, and different embryo developmental potentials between early and late stage spermatids, providing evidence that maturation of round spermatids impacts on embryo development. This work provides valuable insights into mammalian spermatogenesis, and a comprehensive resource for future studies towards the complete elucidation of gametogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.