ArticleScientific reports2018
Discovery and control of culturable and viable but non-culturable cells of a distinctive Lactobacillus harbinensis strain from spoiled beer.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Bioelectrochemical detection of viable but non-culturable bacterial cells on screen-printed carbon electrodes.Biofilm · 2026Article
- Molecular identification of lactic acid bacteria from traditional fermented foods and screening exopolysaccharide production by using food wastes.Folia microbiologica · 2025Article
- Resistance to preservatives and the viable but non-culturable state formation ofFrontiers in microbiology · 2024Article
- The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria.Microorganisms · 2023Review
- Lactic acid bacteria biofilms and their antimicrobial potential against pathogenic microorganisms.Biofilm · 2023Review
- Uncultured Microorganisms and Their Functions in the Fermentation Systems of Traditional Chinese Fermented Foods.Foods (Basel, Switzerland) · 2023Review
- Biofilm formation of two genetically diverseFrontiers in microbiology · 2023Article
- Methods for detection and identification of beer-spoilage microbes.Frontiers in microbiology · 2023Review
- Beer Safety: New Challenges and Future Trends within Craft and Large-Scale Production.Foods (Basel, Switzerland) · 2022Review
- Formic acid, an organic acid food preservative, induces viable-but-non-culturable state, and triggers new Antimicrobial Resistance traits inFrontiers in microbiology · 2022Article
- Development of bioactive solid support for immobilized Lactobacillus casei biofilms and the production of lactic acid.Bioprocess and biosystems engineering · 2022Article
- In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonicBioengineered · 2021Article
- Disinfecting handheld electronic devices with UV-C in a healthcare setting.Infection prevention in practice · 2021Article
- Bioprocessing and integration of a high flux screening systematic platform based on isothermal amplification for the detection on 8 common pathogens.Bioprocess and biosystems engineering · 2021Article
- Characterization on gut microbiome of PCOS rats and its further design by shifts in high-fat diet and dihydrotestosterone induction in PCOS rats.Bioprocess and biosystems engineering · 2021Article
- Induction and Resuscitation of Viable but NonculturableMicroorganisms · 2021Article
- How to Evaluate Non-Growing Cells-Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- Systematic Understanding of the Mechanism of Baicalin against Gastric Cancer Using Transcriptome Analysis.BioMed research international · 2021Article
- Pathogenic and Virulence Factor Detection on Viable but Non-culturable Methicillin-ResistantFrontiers in microbiology · 2021Article
- Morphological and physiological changes in Lentilactobacillus hilgardii cells after cold plasma treatment.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Occasional beer spoilage incidents caused by false-negative isolation of lactic acid bacteria (LAB) in the viable but non-culturable (VBNC) state, result in significant profit loss and pose a major concern in the brewing industry. In this study, both culturable and VBNC cells of an individual Lactobacillus harbinensis strain BM-LH14723 were identified in one spoiled beer sample by genome sequencing, with the induction and resuscitation of VBNC state for this strain further investigated. Formation of the VBNC state was triggered by low-temperature storage in beer (175 ± 1.4 days) and beer subculturing (25 ± 0.8 subcultures), respectively, and identified by both traditional staining method and PMA-PCR. Resuscitated cells from the VBNC state were obtained by addition of catalase rather than temperature upshift, changing medium concentration, and adding other chemicals, and both VBNC and resuscitated cells retained similar beer-spoilage capability as exponentially growing cells. In addition to the first identification of both culturable and VBNC cells of an individual L. harbinensis strain from spoiled beer, this study also for the first time reported the VBNC induction and resuscitation, as well as verification of beer-spoilage capability of VBNC and resuscitated cells for the L. harbinensis strain. Genes in association with VBNC state were also identified by the first genome sequencing of beer spoilage L. harbinensis. The results derived from this study suggested the contamination and spoilage of beer products by VBNC and resuscitated L. harbinensis strain BM-LH14723.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.