ArticleProceedings of the National Academy of Sciences of the United States of America2018
Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 75 citations in OpenAlex.
- Use of translational fusions to express functional Klebsiella oxytoca dinitrogenase reductase in plant mitochondria.Plant molecular biology · 2026Article
- Enhancement of Nitrogenase Activity in Recombinant Escherichia coli by Replacing Upstream Region of nif Operon from Paenibacillus Species.Chembiochem : a European journal of chemical biology · 2026Article
- Article
- Heterologous Expression of the Nitrogen-Fixing Gene Cluster fromMicroorganisms · 2025Article
- Investigation of themBio · 2025Article
- Multiplex Expression Cassette Assembly: A flexible and versatile method for building complex genetic circuits in conventional vectors.Plant biotechnology journal · 2024Article
- Article
- Engineering Synthetic Electron Transfer Chains from Metallopeptide Membranes.Inorganic chemistry · 2024Article
- Organelle-dependent polyprotein designs enable stoichiometric expression of nitrogen fixation components targeted to mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity.Microbial cell factories · 2023Article
- Article
- Ammonia Production Using Bacteria and Yeast toward a Sustainable Society.Bioengineering (Basel, Switzerland) · 2023Review
- Engineering Nitrogenases for Synthetic Nitrogen Fixation: From Pathway Engineering to Directed Evolution.Biodesign research · 2023Review
- Assembly of nitrogenase biosynthetic pathway inFrontiers in microbiology · 2023Article
- Genes Associated with Biological Nitrogen Fixation Efficiency Identified Using RNA Sequencing in Red Clover (Life (Basel, Switzerland) · 2022Article
- Biological nitrogen fixation and prospects for ecological intensification in cereal-based cropping systems.Field crops research · 2022Review
- A colorimetric method to measure in vitro nitrogenase functionality for engineering nitrogen fixation.Scientific reports · 2022Article
- A critical role of an oxygen-responsive gene for aerobic nitrogenase activity in Azotobacter vinelandii and its application to Escherichia coli.Scientific reports · 2022Article
- Efficient production of protein complexes in mammalian cells using a poxvirus vector.PloS one · 2022Article
- Nitrogen fixation in maize: breeding opportunities.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
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6 authors at 2 institutions in 2 countries.
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Abstract
Re-engineering of complex biological systems (CBS) is an important goal for applications in synthetic biology. Efforts have been made to simplify CBS by refactoring a large number of genes with rearranged polycistrons and synthetic regulatory circuits. Here, a posttranslational protein-splicing strategy derived from RNA viruses was exploited to minimize gene numbers of the classic nitrogenase system, where the expression stoichiometry is particularly important. Operon-based
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