ArticleBiosensors & bioelectronics2019
Integrated phosphorescence-based photonic biosensor (iPOB) for monitoring oxygen levels in 3D cell culture systems.
Article in Biosensors & bioelectronics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Wearable and Implantable Devices for Continuous Monitoring of Muscle Physiological Activity: A Review.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Impacts of Oxygen Tension on Developmental Competence of Preimplantation Embryos.Biomolecules · 2026Review
- Toward continuous monitoring systems: emerging trends of on-chip sensors in organ-on-a-chip.Microsystems & nanoengineering · 2026Review
- 3D Printing Assisted Wearable and Implantable Biosensors.Biosensors · 2025Review
- Recent Advances in Micro- and Nano-Enhanced Intravascular Biosensors for Real-Time Monitoring, Early Disease Diagnosis, and Drug Therapy Monitoring.Sensors (Basel, Switzerland) · 2025Review
- Review
- Integration of secreted signaling molecule sensing on cell monitoring platforms: a critical review.Analytical and bioanalytical chemistry · 2024Review
- Surface Deformation of Biocompatible Materials: Recent Advances in Biological Applications.Biomimetics (Basel, Switzerland) · 2024Review
- The 3D Printing of Nanocomposites for Wearable Biosensors: Recent Advances, Challenges, and Prospects.Bioengineering (Basel, Switzerland) · 2023Review
- Three-dimensional oxygen concentration monitoring in hydrogels using low-cost phosphorescence lifetime imaging for tissue engineering.Biomedical optics express · 2023Article
- Bone/cartilage organoid on-chip: Construction strategy and application.Bioactive materials · 2023Review
- Pollution-Free and Highly Sensitive Lactate Detection in Cell Culture Based on a Microfluidic Chip.Micromachines · 2023Article
- Nanocomposite Hydrogels as Functional Extracellular Matrices.Gels (Basel, Switzerland) · 2023Review
- A new microphysiological system shows hypoxia primes human ISCs for interleukin-dependent rescue of stem cell activity.bioRxiv : the preprint server for biology · 2023Article
- OFrontiers in bioengineering and biotechnology · 2023Article
- Article
- Biosensors to Monitor Cell Activity in 3D Hydrogel-Based Tissue Models.Sensors (Basel, Switzerland) · 2022Review
- Microfluidic-Based Oxygen (OBiosensors · 2021Review
- Advancement of Sensor Integrated Organ-on-Chip Devices.Sensors (Basel, Switzerland) · 2021Review
- Biofilms as Promoters of Bacterial Antibiotic Resistance and Tolerance.Antibiotics (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Physiological processes, such as respiration, circulation, digestion, and many pathologies alter oxygen concentration in the blood and tissue. When designing culture systems to recapitulate the in vivo oxygen environment, it is important to integrate systems for monitoring and controlling oxygen concentration. Herein, we report the design and engineering of a system to remotely monitor and control oxygen concentration inside a device for 3D cell culture. We integrate a photonic oxygen biosensor into the 3D tissue scaffold and regulate oxygen concentration via the control of purging gas flow. The integrated phosphorescence-based oxygen biosensor employs the quenching of palladium-benzoporphyrin by molecular oxygen to transduce the local oxygen concentration in the 3D tissue scaffold. The system is validated by testing the effects of normoxic and hypoxic culture conditions on healthy and tumorigenic breast epithelial cells, MCF-10A cells and BT474 cells, respectively. Under hypoxic conditions, both cell types exhibited upregulation of downstream target genes for the hypoxia marker gene, hypoxia-inducible factor 1α (HIF1A). Lastly, by monitoring the real-time fluctuation of oxygen concentration, we illustrated the formation of hypoxic culture conditions due to limited diffusion of oxygen through 3D tissue scaffolds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.