Evidence map›Paper›PMID 30060747›Full record

ArticleMalaria journal2018

A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharovi.

Gleb N Artemov, Alena I Velichevskaya, Semen M Bondarenko, Gayane H Karagyan, Sargis A Aghayan, Marine S Arakelyan, Vladimir N Stegniy, Igor V Sharakhov, Maria V Sharakhova

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Article in Malaria journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Gleb N ArtemovLaboratory of Ecology, Genetics and Environment Protection, Tomsk State University, Tomsk, Russia.
Alena I VelichevskayaLaboratory of Ecology, Genetics and Environment Protection, Tomsk State University, Tomsk, Russia.
Semen M BondarenkoLaboratory of Ecology, Genetics and Environment Protection, Tomsk State University, Tomsk, Russia.
Gayane H KaragyanScientific Center of Zoology and Hydroecology, The National Academy of Sciences of the Republic of Armenia, Yerevan, Armenia.
Sargis A AghayanScientific Center of Zoology and Hydroecology, The National Academy of Sciences of the Republic of Armenia, Yerevan, Armenia.
Marine S ArakelyanChair of Zoology, Yerevan State University, Yerevan, Armenia.
Vladimir N StegniyLaboratory of Ecology, Genetics and Environment Protection, Tomsk State University, Tomsk, Russia.
Igor V SharakhovLaboratory of Ecology, Genetics and Environment Protection, Tomsk State University, Tomsk, Russia. igor@vt.edu.
Maria V SharakhovaLaboratory of Ecology, Genetics and Environment Protection, Tomsk State University, Tomsk, Russia. msharakh@vt.edu.

Funding

Russian Science Foundation 15-14-20011
6 · The paper itself

Abstract

backgroundAnopheles sacharovi is a dominant malaria vector species in South Europe and the Middle East which has a highly plastic behaviour at both adult and larval stages. Such plasticity has prevented this species from eradication by several anti-vector campaigns. The development of new genome-based strategies for vector control will benefit from genome sequencing and physical chromosome mapping of this mosquito. Although a cytogenetic photomap for chromosomes from salivary glands of An. sacharovi has been developed, no cytogenetic map suitable for physical genome mapping is available.

methodsMosquitoes for this study were collected at adult stage in animal shelters in Armenia. Polytene chromosome preparations were prepared from ovarian nurse cells. Fluorescent in situ hybridization (FISH) was performed using PCR amplified probes.

resultsThis study constructed a high-quality standard photomap for polytene chromosomes from ovarian nurse cells of An. sacharovi. Following the previous nomenclature, chromosomes were sub-divided into 39 numbered and 119 lettered sub-divisions. Chromosomal landmarks for the chromosome recognition were described. Using FISH, 4 PCR-amplified genic probes were mapped to the chromosomes. The positions of the probes demonstrated gene order reshuffling between An. sacharovi and Anopheles atroparvus which has not been seen cytologically. In addition, this study described specific chromosomal landmarks that can be used for the cytotaxonomic diagnostics of An. sacharovi based on the banding pattern of its polytene chromosomes.

conclusionsThis study constructed a high-quality standard photomap for ovarian nurse cell chromosomes of An. sacharovi and validated its utility for physical genome mapping. Based on the map, cytotaxonomic features for identification of An. sacharovi have been described. The cytogenetic map constructed in this study will assist in creating a chromosome-based genome assembly for this mosquito and in developing cytotaxonomic tools for identification of other species from the Maculipennis group.

Indexed as

Chromosome MappingPolytene ChromosomesAnimalsAnophelesIn Situ Hybridization, FluorescenceMalariaMosquito VectorsSalivary GlandsAnopheles sacharoviFluorescence in situ hybridizationGytogenetic mapMosquito

Identifiers

PMID30060747
PMCPMC6065146

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.