Evidence map›Paper›PMID 30059978›Full record

ArticleEndocrinology2018

A Genetic Interaction Map of Insulin Production Identifies Mfi as an Inhibitor of Mitochondrial Fission.

Jessica Lee, Zachary Pappalardo, Deeksha Gambhir Chopra, Thomas G Hennings, Ian Vaughn, Christopher Lan, Justin J Choe, Kenny Ang, Steven Chen, Michelle Arkin and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 87% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Jessica LeeDiabetes Center, University of California, San Francisco, San Francisco, California.
Zachary PappalardoDiabetes Center, University of California, San Francisco, San Francisco, California.
Deeksha Gambhir ChopraDiabetes Center, University of California, San Francisco, San Francisco, California.
Thomas G HenningsDiabetes Center, University of California, San Francisco, San Francisco, California.
Ian VaughnDiabetes Center, University of California, San Francisco, San Francisco, California.
Christopher LanDiabetes Center, University of California, San Francisco, San Francisco, California.
Justin J ChoeDiabetes Center, University of California, San Francisco, San Francisco, California.
Kenny AngSmall Molecules Discovery Center, University of California, San Francisco, San Francisco, California.
Steven ChenSmall Molecules Discovery Center, University of California, San Francisco, San Francisco, California.
Michelle ArkinSmall Molecules Discovery Center, University of California, San Francisco, San Francisco, California.
Michael T McManusDiabetes Center, University of California, San Francisco, San Francisco, California.
Michael S GermanDiabetes Center, University of California, San Francisco, San Francisco, California.
Gregory M KuDiabetes Center, University of California, San Francisco, San Francisco, California.
University of California, San Francisco · US

Funding

Research BaseP30DK063720 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GERMAN, MICHAEL S · 2003 to 2019
$21.8M
DIABETES, ENDOCRINOLOGY &METABOLISM TRAINING PROGRAMT32DK007418 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI EDWARD C HSIAO · 1986 to 2026
$14.3M
The role of Spry2 in beta cell function and the unfolded protein responseR01DK107650 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KU, GREGORY MICHAEL · 2016 to 2020
$1.8M
Whole genome RNA-interference screen for novel regulators of the insulin promoterRC1DK086290 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MCMANUS, MICHAEL T · 2009 to 2010
$995k
Discovering and dissecting new regulators of insulin production in beta cellsK08DK087945 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KU, GREGORY MICHAEL · 2011 to 2015
$747k
A novel, beta cell specific regulator of the insulin promoterR03DK102892 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KU, GREGORY MICHAEL · 2014 to 2015
$158k
NIDDK NIH HHS K08 DK087945NIDDK NIH HHS P30 DK063720NIDDK NIH HHS R01 DK107650NIDDK NIH HHS R03 DK102892NIDDK NIH HHS RC1 DK086290NIDDK NIH HHS T32 DK007418
6 · The paper itself

Abstract

Insulin production by the pancreatic β cell is critical for the glucose homeostasis of the whole organism. Although the transcription factors required for insulin production are known, the upstream pathways that control insulin production are less clear. To further elucidate this regulatory network, we created a genetic interaction map of insulin production by performing ∼20,000 pairwise RNA interference knockdowns of insulin promoter regulators. Our map correctly predicted known physical complexes in the electron transport chain and a role for Spry2 in the unfolded protein response. To further validate our map, we used it to predict the function of an unannotated gene encoding a 37-kDa protein with no identifiable domains we have termed mitochondrial fission factor interactor (Mfi). We have shown that Mfi is a binding partner of the mitochondrial fission factor and that Mfi inhibits dynamin-like protein 1 recruitment to mitochondria. Our data provide a resource to understand the regulatory network of insulin promoter activity.

Indexed as

AnimalsCell LineDynaminsGene Regulatory NetworksGTP PhosphohydrolasesHumansInsulinInsulin-Secreting CellsIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceMicrotubule-Associated ProteinsMitochondriaMitochondrial DynamicsMitochondrial ProteinsPromoter Regions, GeneticDNM1L protein, humanDynaminsGTP PhosphohydrolasesInsulinIntracellular Signaling Peptides and ProteinsMembrane ProteinsMff protein, humanMicrotubule-Associated ProteinsMitochondrial ProteinsSPRY2 protein, human

Identifiers

PMID30059978
PMCPMC6112596
OpenAlexW2883106416

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.