ArticleJournal of visualized experiments : JoVE2018
Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Methodological considerations in the analysis of guard cell starch metabolism.The New phytologist · 2026Article
- Cell-type-specific compartmentalization and function of the glucosinolate-myrosinase system in Arabidopsis thaliana.The Journal of biological chemistry · 2026Article
- Guard cell photorespiration controls stomata behavior and development.The New phytologist · 2026Article
- Guard cells on the adaxial and abaxial leaf surfaces use different compositions of potassium ion channels to drive light-induced stomatal opening.Nature plants · 2025Article
- Guard cell-specific glycine decarboxylase manipulation affects Arabidopsis photosynthesis, growth and stomatal behavior.The New phytologist · 2025Article
- Hydrogen peroxide is required for light-induced stomatal opening across different plant species.Nature communications · 2024Article
- Cell size differences affect photosynthetic capacity in a Mesoamerican and an Andean genotype ofFrontiers in plant science · 2024Article
- Role ofPlants (Basel, Switzerland) · 2023Article
- TOR promotes guard cell starch degradation by regulating the activity of β-AMYLASE1 in Arabidopsis.The Plant cell · 2022Article
- Identification of stomatal-regulating molecules from de novo arylamine collection through aromatic C-H amination.Scientific reports · 2022Article
- Article
- Guard Cell-Specific Pectin METHYLESTERASE53 Is Required for Abscisic Acid-Mediated Stomatal Function and Heat Response inFrontiers in plant science · 2022Article
- Loss of Arabidopsis matrix metalloproteinase-5 affects root development and root bacterial communities during drought stress.Physiologia plantarum · 2021Article
- The Plastid-Localized AtFtsHi3 Pseudo-Protease ofFrontiers in plant science · 2021Article
- Proteomics and phosphoproteomics revealed molecular networks of stomatal immune responses.Planta · 2020Article
- Molecular changes in Mesembryanthemum crystallinum guard cells underlying the CPlant molecular biology · 2020Article
- Brassinosteroid and Hydrogen Peroxide Interdependently Induce Stomatal Opening by Promoting Guard Cell Starch Degradation.The Plant cell · 2020Article
- S-Nitroso-Proteome Revealed in Stomatal Guard Cell Response to Flg22.International journal of molecular sciences · 2020Article
- Physiological Changes inFrontiers in plant science · 2020Article
- Acclimation and hardening of a slow-growing woody species emblematic to western North America from in vitro plantlets.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of guard cells is essential to the knowledge of the specific contributions of this cell type to the overall function of plants. However, it is often difficult to isolate and thus studying them often provides a challenge. This study establishes a stomatal guard cell enrichment method. The protocol utilizes Scotch tape to isolate guard cells and extract proteins from the cells. This method improves the integrity and yield of guard cell during isolation and provides a reliable sample for the study of cell signaling processes and how they correlate to stomatal movement phenotypes. In this method, the plant leaves were partitioned between two portions of tape and peeled apart to remove the abaxial side. This protocol was applied to Arabidopsis leaf tissue to isolate guard cells. The cells were treated with fluorescein diacetate (FDA) to determine viability and with abscisic acid (ABA) to assess stomata movement in response to ABA. In conclusion, this protocol proves useful for preparing enriched stomatal guard cells and isolating proteins. The quality of the cells and proteins obtained here enable physiological and other biological studies.
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Registered trials
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