ArticlePlant biotechnology journal2019
Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Nicotiana hairy roots for recombinant protein expression, where to start? A systematic review.Molecular biology reports · 2023Pooled it
- Plant molecular farming: a promising frontier for orphan drug production.Biotechnology letters · 2025Review
- Comparison of silver and gold nanoparticles green synthesis by Artemisia annua hairy root extracts.Biology open · 2025Article
- The Untapped Potential of Hairy Root Cultures and Their Multiple Applications.International journal of molecular sciences · 2024Review
- Artificial intelligence-driven systems engineering for next-generation plant-derived biopharmaceuticals.Frontiers in plant science · 2023Review
- Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process.Biotechnology and bioengineering · 2022Article
- Investigation of theMolecules (Basel, Switzerland) · 2022Article
- Compendium on Food Crop Plants as a Platform for Pharmaceutical Protein Production.International journal of molecular sciences · 2022Review
- Hairy roots: An untapped potential for production of plant products.Frontiers in plant science · 2022Review
- Determination of the Qualitative Composition of Biologically-Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal PlantBiomolecules · 2021Article
- Plant Platforms for Efficient Heterologous Protein Production.Biotechnology and bioprocess engineering : BBE · 2021Review
- Arabidopsis Hairy Roots Producing High Level of Active Human Gastric Lipase.Molecular biotechnology · 2020Article
- Hairy Root Cultures-A Versatile Tool With Multiple Applications.Frontiers in plant science · 2020Review
- High Efficient Expression and Purification of Human Epidermal Growth Factor inInternational journal of molecular sciences · 2019Article
- Assessing Carnivorous Plants for the Production of Recombinant Proteins.Frontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The Brassica rapa hairy root based expression platform, a turnip hairy root based expression system, is able to produce human complex glycoproteins such as the alpha-L-iduronidase (IDUA) with an activity similar to the one produced by Chinese Hamster Ovary (CHO) cells. In this article, a particular attention has been paid to the N- and O-glycosylation that characterize the alpha-L-iduronidase produced using this hairy root based system. This analysis showed that the recombinant protein is characterized by highly homogeneous post translational profiles enabling a strong batch to batch reproducibility. Indeed, on each of the 6 N-glycosylation sites of the IDUA, a single N-glycan composed of a core Man
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.