ArticlePharmaceutical biology2018
Assessing product adulteration of Eurycoma longifolia (Tongkat Ali) herbal medicinal product using DNA barcoding and HPLC analysis.
Article in Pharmaceutical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Authentication ofFrontiers in pharmacology · 2022Pooled it
- Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.Phytochemical analysis : PCA · 2025Review
- Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds inPlants (Basel, Switzerland) · 2023Review
- A Multifaceted Review ofACS omega · 2023Review
- DNA Barcoding Medicinal Plant Species from Indonesia.Plants (Basel, Switzerland) · 2022Article
- Article
- Integrated Approach for Species Identification and Quality Analysis forPlants (Basel, Switzerland) · 2021Article
- Chemical Authentication of Botanical Ingredients: A Review of Commercial Herbal Products.Frontiers in pharmacology · 2021Review
- Pitfalls and promises of raw drug identification techniques in the ayurvedic industry: an overview.3 Biotech · 2020Review
- Mitigating the Impact of Admixtures in Thai Herbal Products.Frontiers in pharmacology · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
contextEurycoma longifolia Jack (Simaroubaceae) commonly known as Tongkat Ali is one of the most important plants in Malaysia. The plant extracts (particularly roots) are widely used for the treatment of cough and fever besides having antimalarial, antidiabetic, anticancer and aphrodisiac activities.
objectivesThis study assesses the extent of adulteration of E. longifolia herbal medicinal products (HMPs) using DNA barcoding validated by HPLC analysis. MATERIALS AND
methodsChloroplastic rbcL and nuclear ITS2 barcode regions were used in the present study. The sequences generated from E. longifolia HMPs were compared to sequences in the GenBank using MEGABLAST to verify their taxonomic identity. These results were verified by neighbor-joining tree analysis in which branches of unknown specimen are compared to the reference sequences established from this study and other retrieved from the GenBank. The HMPs were also analysed using HPLC analysis for the presence of eurycomanone bioactive marker.
resultsIdentification using DNA barcoding revealed that 37% of the tested HMPs were authentic while 27% were adulterated with the ITS2 barcode region proven to be the ideal marker. The validation of the authenticity using HPLC analysis showed a situation in which a species which was identified as authentic was found not to contain the expected chemical compound. DISCUSSION AND
conclusionsDNA barcoding should be used as the first screening step for testing of HMPs raw materials. However, integration of DNA barcoding with HPLC analysis will help to provide detailed knowledge about the safety and efficacy of the HMPs.
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