ArticleLiver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society2018
Combined Hypothermic and Normothermic Machine Perfusion Improves Functional Recovery of Extended Criteria Donor Livers.
Article in Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 122 citations in OpenAlex.
- Association between Hepatocellular Carcinoma Recurrence and Graft Size in Living Donor Liver Transplantation: A Systematic Review.International journal of molecular sciences · 2023Pooled it
- Portal-Venous Hypothermic Oxygenated Perfusion for Liver Transplant: A Randomized Clinical Trial.JAMA surgery · 2026Trial
- Revealing the potential of non-transplanted donor organs: a comparative analysis in the Eurotransplant region.Transplant international : official journal of the European Society for Organ Transplantation · 2026Article
- Graft quality assessment during machine perfusion in liver transplantation: a review of current evidence and emerging strategies.Frontiers in transplantation · 2026Review
- The Role of Machine Perfusion for Liver Transplantation in Children.Pediatric transplantation · 2025Review
- Ex-Situ Dual Hypothermic Oxygenated Machine Perfusion in Full-Left-Full-Right Split Liver Transplantation for Two Adult Recipients.Journal of clinical medicine · 2025Article
- 'Back-to-base' combined hypothermic and normothermic machine perfusion of human donor livers.Nature protocols · 2025Review
- From critical care to organ preservation: the potential role of hemadsorption in transplantation-a narrative review.Hepatobiliary surgery and nutrition · 2025Review
- Equal performance of HTK-based and UW-based perfusion solutions in sub-normothermic liver machine perfusion.Scientific reports · 2025Article
- [Advances and perspectives in vascularized composite allotransplantation preservation].Bulletin de l'Academie nationale de medecine · 2024Article
- Distinctive protein expression in elderly livers in a Sprague-Dawley rat model of normothermic ex vivo liver machine perfusion.European journal of medical research · 2024Article
- The Interplay between Perioperative Oxidative Stress and Hepatic Dysfunction after Human Liver Resection: A Prospective Observational Pilot Study.Antioxidants (Basel, Switzerland) · 2024Article
- Modification of Preservative Fluids with Antioxidants in Terms of Their Efficacy in Liver Protection before Transplantation.International journal of molecular sciences · 2024Review
- Establishing a HOPE Program in a Real-life Setting: A Brazilian Case Series.Transplantation direct · 2023Article
- Comparison of in-gel and in-solution proteolysis in the proteome profiling of organ perfusion solutions.Clinical proteomics · 2023Article
- Extended criteria brain-dead organ donors: Prevalence and impact on the utilisation of livers for transplantation in Brazil.World journal of hepatology · 2023Article
- Pushing the boundaries of innovation: the potential ofFrontiers in cardiovascular medicine · 2023Review
- Solid Organ Donation and Transplantation in the United Kingdom: Good Governance is Key to Success.Transplant international : official journal of the European Society for Organ Transplantation · 2023Review
- Current practice and novel approaches in organ preservation.Frontiers in transplantation · 2023Review
- Changing liver utilization and discard rates in clinical transplantation in the ex-vivo machine preservation era.Frontiers in medical technology · 2023Review
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
Abstract
Hypothermic oxygenated perfusion (HOPE) and normothermic perfusion are seen as distinct techniques of ex situ machine perfusion of the liver. We aimed to demonstrate the feasibility of combining both techniques and whether it would improve functional parameters of donor livers into transplant standards. Ten discarded human donor livers had either 6 hours of normothermic perfusion (n = 5) or 2 hours of HOPE followed by 4 hours of normothermic perfusion (n = 5). Liver function was assessed according to our viability criteria; markers of tissue injury and hepatic metabolic activity were compared between groups. Donor characteristics were comparable. During the hypothermic perfusion phase, livers down-regulated mitochondrial respiration (oxygen uptake, P = 0.04; partial pressure of carbon dioxide perfusate, P = 0.04) and increased adenosine triphosphate levels 1.8-fold. Following normothermic perfusion, those organs achieved lower tissue expression of markers of oxidative injury (4-hydroxynonenal, P = 0.008; CD14 expression, P = 0.008) and inflammation (CD11b, P = 0.02; vascular cell adhesion molecule 1, P = 0.05) compared with livers that had normothermic perfusion alone. All livers in the combined group achieved viability criteria, whereas 40% (2/5) in the normothermic group failed (P = 0.22). In conclusion, this study suggests that a combined protocol of hypothermic oxygenated and normothermic perfusions might attenuate oxidative stress, tissue inflammation, and improve metabolic recovery of the highest-risk donor livers compared with normothermic perfusion alone.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.