ArticleApplied physics letters2018
Imaging the three-dimensional orientation and rotational mobility of fluorescent emitters using the Tri-spot point spread function.
Article in Applied physics letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Harnessing fluorescent probes for molecular diagnosis and theranostics of atherosclerosis.Drug delivery · 2026Review
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Fused deep learning enables 6D single-molecule localization in polarization-resolved microscopy.Methods and applications in fluorescence · 2026Article
- 4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.Nature communications · 2026Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- POLCAM: instant molecular orientation microscopy for the life sciences.Nature methods · 2024Article
- Single-molecule electrochemical imaging resolves the midpoint potentials of individual fluorophores on nanoporous antimony-doped tin oxide.Chemical science · 2024Article
- Combining deep learning approaches and point spread function engineering for simultaneous 3D position and 3D orientation measurements of fluorescent single molecules.Optics communications · 2023Article
- Six-Dimensional Single-Molecule Imaging with Isotropic Resolution using a Multi-View Reflector Microscope.Nature photonics · 2023Article
- Deep-SMOLM: deep learning resolves the 3D orientations and 2D positions of overlapping single molecules with optimal nanoscale resolution.Optics express · 2022Article
- Photon efficient orientation estimation using polarization modulation in single-molecule localization microscopy.Biomedical optics express · 2022Article
- Resolving the Three-Dimensional Rotational and Translational Dynamics of Single Molecules Using Radially and Azimuthally Polarized Fluorescence.Nano letters · 2022Article
- 4polar-STORM polarized super-resolution imaging of actin filament organization in cells.Nature communications · 2022Article
- Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function.The journal of physical chemistry. B · 2021Article
- Simultaneous orientation and 3D localization microscopy with a Vortex point spread function.Nature communications · 2021Article
- The final twist in endocytic membrane scission.Nature cell biology · 2021Article
- 3D printable diffractive optical elements by liquid immersion.Nature communications · 2021Article
- Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence.Biomedical optics express · 2021Article
- Birefringent Fourier filtering for single molecule coordinate and height super-resolution imaging with dithering and orientation.Nature communications · 2020Article
- Single-Molecule 3D Orientation Imaging Reveals Nanoscale Compositional Heterogeneity in Lipid Membranes.Angewandte Chemie (International ed. in English) · 2020Article
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Authors and funding
4 authors.
Funding
Abstract
Fluorescence photons emitted by single molecules contain rich information regarding their rotational motions, but adapting single-molecule localization microscopy (SMLM) to measure their orientations and rotational mobilities with high precision remains a challenge. Inspired by dipole radiation patterns, we design and implement a Tri-spot point spread function (PSF) that simultaneously measures the three-dimensional orientation and the rotational mobility of dipole-like emitters across a large field of view. We show that the orientation measurements done using the Tri-spot PSF are sufficiently accurate to correct the anisotropy-based localization bias, from 30 nm to 7 nm, in SMLM. We further characterize the emission anisotropy of fluorescent beads, revealing that both 20-nm and 100-nm diameter beads emit light significantly differently from isotropic point sources. Exciting 100-nm beads with linearly polarized light, we observe significant depolarization of the emitted fluorescence using the Tri-spot PSF that is difficult to detect using other methods. Finally, we demonstrate that the Tri-spot PSF detects rotational dynamics of single molecules within a polymer thin film that are not observable by conventional SMLM.
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