Evidence map›Paper›PMID 30057299›Full record

ArticleCell chemical biology2018

Real-Time Ligand Binding of Fluorescent VEGF-A Isoforms that Discriminate between VEGFR2 and NRP1 in Living Cells.

Chloe J Peach, Laura E Kilpatrick, Rachel Friedman-Ohana, Kris Zimmerman, Matthew B Robers, Keith V Wood, Jeanette Woolard, Stephen J Hill

Open access · hybridAbstract read
In one paragraph

Article in Cell chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 42 citations in OpenAlex.

  1. Article
  2. Article
  3. A method for rapid and reliable quantification of VEGF-cell binding activity.Biochemical and biophysical research communications · 2024
    Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Serine-arginine-rich protein kinase-1 inhibition for the treatment of diabetic retinopathy.American journal of physiology. Heart and circulatory physiology · 2022
    Article
  10. Interactions between Ligand-Bound EGFR and VEGFR2.Journal of molecular biology · 2021
    Article
  11. Article
  12. Article
  13. Complex Formation between VEGFR2 and the βCell chemical biology · 2019
    Article
  14. Article
  15. Article
  16. Binding kinetics of ligands acting at GPCRs.Molecular and cellular endocrinology · 2019
    Review
  17. NanoBRET: The Bright Future of Proximity-Based Assays.Frontiers in bioengineering and biotechnology · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Chloe J PeachDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, Nottingham NG7 2UH, UK.
Laura E KilpatrickDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, Nottingham NG7 2UH, UK.
Rachel Friedman-OhanaPromega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.
Kris ZimmermanPromega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.
Matthew B RobersPromega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.
Keith V WoodPromega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.
Jeanette WoolardDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, Nottingham NG7 2UH, UK. Electronic address: jeanette.woolard@nottingham.ac.uk.
Stephen J HillDivision of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, Nottingham NG7 2UH, UK. Electronic address: stephen.hill@nottingham.ac.uk.
Promega (United States) · USUniversity of Nottingham · GB

Funding

Biotechnology and Biological Sciences Research Council BB/L019418/1Medical Research Council MR/N020081/1
6 · The paper itself

Abstract

Fluorescent VEGF-A isoforms have been evaluated for their ability to discriminate between VEGFR2 and NRP1 in real-time ligand binding studies in live cells using BRET. To enable this, we synthesized single-site (N-terminal cysteine) labeled versions of VEGF

Indexed as

Energy TransferFluorescent DyesHEK293 CellsHumansHuman Umbilical Vein Endothelial CellsLigandsLuminescent MeasurementsNeuropilin-1Protein BindingProtein IsoformsRhodaminesVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2Fluorescent DyesLigandsNeuropilin-1NRP1 protein, humanProtein IsoformsRhodaminestetramethylrhodamineVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2VEGFA protein, humanligand binding kineticsNanoBRETneuropilin-1receptor mechanismsVEGF isoformsVEGFR2

Identifiers

PMID30057299
PMCPMC6200776
OpenAlexW2883934520

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.