ArticleAsian-Australasian journal of animal sciences2019
Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers.
Article in Asian-Australasian journal of animal sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- SuSha: A multi-model ensemble learning framework for predicting microbial salinity adaptation.Engineering microbiology · 2026Article
- Lyophilized rumen fluid from goats fed different roughage-based diets: fermentation characteristics, microbial communities, and post-reconstitutionFrontiers in microbiology · 2026Article
- Methyl coenzyme M reductase as a target for inhibition of methanogenesis in ruminants: challenges and opportunities.Frontiers in microbiology · 2025Review
- Holistic View and Novel Perspective on Ruminal and Extra-Gastrointestinal Methanogens in Cattle.Microorganisms · 2023Review
- Evolving understanding of rumen methanogen ecophysiology.Frontiers in microbiology · 2023Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
objectiveTo isolate and identify new methanogens from the rumen of Holstein steers in Korea.
methodsRepresentative rumen contents were obtained from three ruminally cannulated Holstein steers (793±8 kg). Pre-reduced media were used for the growth and isolation of methanogens. Optimum growth temperature, pH, and sodium chloride (NaCl) concentration as well as substrate utilization and antibiotic tolerance were investigated to determine the physiological characteristics of the isolated strain. Furthermore, the isolate was microscopically studied for its morphology. Polymerase chain reaction of 16S rRNA and mcrA gene-based amplicons was used for identification.
resultsOne strain designated as KOR-2 was isolated and found to be a non-motile irregular coccus with a diameter of 0.2 to 0.5 μm. KOR-2 utilized H2+CO2 and formate but was unable to metabolize acetate, methanol, trimethylamine, 2-propanol, and isobutanol for growth and methane production. The optimum temperature and pH for the growth of KOR-2 were 38°C and 6.8 to 7.0, respectively, while the optimum NaCl concentration essential for KOR-2 growth was 1.0% (w/v). KOR-2 tolerated ampicillin, penicillin G, kanamycin, spectromycin, and tetracycline. In contrast, the cell growth was inhibited by chloramphenicol. Phylogenetic analysis of 16S rRNA and mcrA genes revealed the relatedness between KOR-2 and Methanoculleus bourgensis.
conclusionBased on the physiological and phylogenetic characteristics, KOR-2 was thought to be a new strain within the genus Methanoculleus and named Methanoculleus bourgensis KOR-2.
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