Evidence map›Paper›PMID 30056590›Full record

ArticleMikrochimica acta2018

Signal amplification by strand displacement in a carbon dot based fluorometric assay for ATP.

Jieping Luo, Xin Shen, Bingzhi Li, Xiaoyun Li, Xuemin Zhou

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 36 citations in OpenAlex.

  1. Review
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  7. A molecular device: A DNA molecular lock driven by the nicking enzymes.Computational and structural biotechnology journal · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jieping LuoSchool of Pharmacy, Nanjing Medical University, Nanjing, 211166, Jiangsu, China.
Xin ShenSchool of Pharmacy, Nanjing Medical University, Nanjing, 211166, Jiangsu, China.
Bingzhi LiSchool of Pharmacy, Nanjing Medical University, Nanjing, 211166, Jiangsu, China.
Xiaoyun LiSchool of Pharmacy, Nanjing Medical University, Nanjing, 211166, Jiangsu, China.
Xuemin ZhouSchool of Pharmacy, Nanjing Medical University, Nanjing, 211166, Jiangsu, China. xueminzhou001_001@hotmail.com.ORCID 0000-0001-7717-0436
Nanjing Medical University · CN

Funding

National Natural Science Foundation of China 21175070National Natural Science Foundation of China 81273480National Natural Science Foundation of China 81572081National Natural Science Foundation of China 81773681
6 · The paper itself

Abstract

A fluorometric ATP assay is described that makes use of carbon dots and graphene oxide along with toehold-mediated strand displacement reaction. In the absence of target, the fluorescence of carbon dots (with excitation/emission maxima at 360/447 nm) is strong and in the "on" state, because the signal probe hybridizes with the aptamer strand and cannot combine with graphene oxide. In the presence of ATP, it will bind to the aptamer and induce a strand displacement reaction. Consequently, the signal probe is released, the sensing strategy will change into the "off" state with the addition of graphene oxide. This aptasensor exhibits selective and sensitive response to ATP and has a 3.3 nM detection limit. Graphical abstract Schematic of signal amplification by strand displacement in a carbon dot based fluorometric assay for ATP. This strategy exhibits high sensitivity and selectivity with a detection limit as low as 3.3 nM.

Indexed as

Adenosine TriphosphateAptamers, NucleotideBiosensing TechniquesCarbonFeasibility StudiesFluorometryGraphiteHumansLimit of DetectionNucleic Acid HybridizationOxidesQuantum DotsAdenosine TriphosphateAptamers, NucleotideCarbonGraphiteOxidesAptamerCarbon dotsFluorescenceGraphene oxideSignal amplification

Identifiers

PMID30056590
OpenAlexW2884749211

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.