Evidence map›Paper›PMID 30055575›Full record

ArticleBMC developmental biology2018

Effects of sex on response of the bovine preimplantation embryo to insulin-like growth factor 1, activin A, and WNT7A.

Paula Tríbulo, Gulnur Jumatayeva, Khoboso Lehloenya, James I Moss, Veronica M Negrón-Pérez, Peter J Hansen

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC developmental biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 3 countries.

Paula TríbuloDepartment of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, PO Box 110910, Gainesville, FL, 32611-0910, USA.
Gulnur JumatayevaDepartment of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, PO Box 110910, Gainesville, FL, 32611-0910, USA.
Khoboso LehloenyaDepartment of Agriculture, University of Zululand, KwaDlangez, Wa 3886, South Africa.
James I MossDepartment of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, PO Box 110910, Gainesville, FL, 32611-0910, USA.
Veronica M Negrón-PérezDepartment of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, PO Box 110910, Gainesville, FL, 32611-0910, USA.
Peter J HansenDepartment of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, PO Box 110910, Gainesville, FL, 32611-0910, USA. pjhansen@ufl.edu.ORCID 0000-0003-3061-9333
University of Florida · USUniversity of Zululand · ZA

Funding

University of Miami Developmental Center for AIDS Research (D-CFAR)P30AI073961 · NIAID · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI GWENDOLYN Barbara SCOTT · 2007 to 2026
$33.8M
Sexual dimorphism in developmental programming caused by CSF2R01HD088352 · NICHD · UNIVERSITY OF FLORIDA · PI HANSEN, PETER J. · 2017 to 2021
$1.8M
Sexual dimorphism in embryo responses to maternal regulatory factorsR03HD080855 · NICHD · UNIVERSITY OF FLORIDA · PI HANSEN, PETER J. · 2014 to 2015
$137k
Eunice Kennedy Shriver National Institute of Child Health and Human Development R01 HD088352Eunice Kennedy Shriver National Institute of Child Health and Human Development R03 HD080855National Institute of Food and Agriculture 2011-67015-30688NIAID NIH HHS P30 AI073961NICHD NIH HHS R01 HD088352NICHD NIH HHS R03 HD080855
6 · The paper itself

Abstract

backgroundAlterations in maternal environment can sometimes affect embryonic development in a sexually-dimorphic manner. The objective was to determine whether preimplantation bovine embryos respond to three maternally-derived cell signaling molecules in a sex-dependent manner.

resultsActions of three embryokines known to increase competence of bovine embryos to develop to the blastocyst stage, insulin-like growth factor 1 (IGF1), activin A, and WNT member 7A (WNT7A), were evaluated for actions on embryos produced in vitro with X- or Y- sorted semen from the same bull. Each embryokine was tested in embryos produced by in vitro fertilization of groups of oocytes with either pooled sperm from two bulls or with sperm from individual bulls. Embryos were treated with IGF1, activin A, or WNT7A on day 5 of culture. All three embryokines increased the proportion of cleaved zygotes that developed to the blastocyst stage and the effect was similar for female and male embryos. As an additional test of sexual dimorphism, effects of IGF1 on blastocyst expression of a total of 127 genes were determined by RT-qPCR using the Fluidigm Delta Gene assay. Expression of 18 genes was affected by sex, expression of 4 genes was affected by IGF1 and expression of 3 genes was affected by the IGF1 by sex interaction.

conclusionSex did not alter how IGF1, activin A or WNT7A altered developmental competence to the blastocyst stage. Thus, sex-dependent differences in regulation of developmental competence of embryos by maternal regulatory signals is not a general phenomenon. The fact that sex altered how IGF1 regulates gene expression is indicative that there could be sexual dimorphism in embryokine regulation of some aspects of embryonic function other than developmental potential to become a blastocyst.

Indexed as

Sex CharacteristicsAnimalsBlastocystCattleFemaleGene Expression Regulation, DevelopmentalHumansInhibin-beta SubunitsInsulin-Like Growth Factor IMaleWnt Proteinsinhibin beta A subunitInhibin-beta SubunitsInsulin-Like Growth Factor IWNT7A protein, humanWnt ProteinsActivin aEmbryoEmbryokineInsulin-like growth factor 1Sex-sorted semenWNT7A

Identifiers

PMID30055575
PMCPMC6064047
OpenAlexW2884595216

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.