Evidence map›Paper›PMID 30055567›Full record

ArticleBMC microbiology2018

Construction of Lactobacillus casei ghosts by Holin-mediated inactivation and the potential as a safe and effective vehicle for the delivery of DNA vaccines.

Rui Hou, Muzi Li, Tingting Tang, Ruichong Wang, Yijing Li, Yigang Xu, Lijie Tang, Li Wang, Min Liu, Yanping Jiang and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 21 citations in OpenAlex.

  1. Frontiers in immunology · 2026
    Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Carbon dots labeledFrontiers in bioengineering and biotechnology · 2023
    Article
  8. Frontiers in immunology · 2022
    Article
  9. Review
  10. Review
  11. Review
  12. DNA Vaccines-How Far From Clinical Use?International journal of molecular sciences · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Rui HouHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Muzi LiHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Tingting TangHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Ruichong WangInstitute for Radiological Protection, Heilongjiang Province Center for Disease Control and Prevention, 40 Youfang Street, Harbin, 150030, China.
Yijing LiHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Yigang XuHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Lijie TangHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Li WangHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Min LiuHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Yanping JiangHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Wen CuiHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China.
Xinyuan QiaoHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China. qiaoxinyuan@126.com.
Northeast Agricultural University · CNHeilongjiang Center for Tuberculosis Control and Prevention · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBacterial ghosts (BGs) are empty bacterial cell envelopes generated by releasing the cellular contents. In this study, a phage infecting Lactobacillus casei ATCC 393 (L. casei 393) was isolated and designated Lcb. We aimed at using L. casei 393 as an antigen delivery system to express phage-derived holin for development of BGs.

resultsA gene fragment encoding holin of Lcb (hocb) was amplified by polymerase chain reaction (PCR). We used L. casei 393 as an antigen delivery system to construct the recombinant strain pPG-2-hocb/L. casei 393. Then the recombinants were induced to express hocb. The immunoreactive band corresponding to hocb was observed by western-blotting, demonstrating the efficiency and specificity of hocb expression in recombinants. The measurements of optical density at 600 nm (OD

conclusionsOur study constructed L. casei BGs by a novel method, which may be a promising technology for promoting the further application of DNA vaccine, providing experimental data to aid the development of other Gram-positive BGs.

Indexed as

Drug Delivery SystemsBacteriophagesCell MembraneDNAGene ExpressionGenetic VectorsLacticaseibacillus caseiPlasmidsRecombinant ProteinsVaccines, DNAViral ProteinsDNARecombinant ProteinsVaccines, DNAViral ProteinsBacterial ghostDNA vaccineLactobacillus caseiPhage

Identifiers

PMID30055567
PMCPMC6064150
OpenAlexW2884369502

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.