ArticleScientific reports2018
Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Unveiling the phenotypic impact of cryopreservation on adipose derived stem cells: A systematic review.The Indian journal of medical research · 2025Pooled it
- Impact of neoadjuvant chemotherapy on the functionality of adipose-derived mesenchymal stromal cells and their modulatory effects on fibroblasts in oncology patients.Scientific reports · 2026Article
- Oncological Safety of High Hydrostatic Pressure Treatment: Effects on Cancer-Associated Fibroblast-like Transdifferentiation of Adipose Stromal Cells.Current issues in molecular biology · 2026Article
- The effect of cold ischemia time on hypoxia, EMT, and apoptosis pathways in normal colon mucosa.PloS one · 2026Article
- Dedifferentiation and metabolic reprogramming of human adipocytes in the tumor niche triggered by colorectal cancer cells.Biological research · 2025Article
- Effect of Constant Inflammation on In Vitro Expanded Adipose-derived Mesenchymal Stromal Cells.Stem cell reviews and reports · 2025Article
- Response of Primary Human Adipocytes to Fatty Acid Treatment.Journal of cellular and molecular medicine · 2025Article
- USP15-modified ADMSCs-Exo alleviates chondrocyte damage and effectively relieved osteoarthritis by inducing M2 polarization of macrophages through deubiquitinating FOXC1.Journal of orthopaedic surgery and research · 2025Article
- Identifying differentiation markers between dermal fibroblasts and adipose-derived mesenchymal stromal cells (AD-MSCs) in human visceral and subcutaneous tissues using single-cell transcriptomics.Stem cell research & therapy · 2025Article
- Development of hybrid biomicroparticles: cellulose exposing functionalized fusion proteins.Microbial cell factories · 2024Article
- Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications.Frontiers in endocrinology · 2024Review
- Research progress in cell therapy for oral diseases: focus on cell sources and strategies to optimize cell function.Frontiers in bioengineering and biotechnology · 2024Review
- Adipose-Derived Stem Cells to Treat Ischemic Diseases: The Case of Peripheral Artery Disease.International journal of molecular sciences · 2023Review
- Stem cell sources from human biological waste material: a role for the umbilical cord and dental pulp stem cells for regenerative medicine.Human cell · 2023Review
- MiRNA regulated therapeutic potential of the stromal vascular fraction: Current clinical applications - A systematic review.Non-coding RNA research · 2023Review
- Outlook of Adipose-Derived Stem Cells: Challenges to Their Clinical Application in Horses.Veterinary sciences · 2023Review
- Adipose-Derived Mesenchymal Stromal Cells in Basic Research and Clinical Applications.International journal of molecular sciences · 2023Review
- The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells.International journal of molecular sciences · 2021Article
- Adipose-derived stromal cells for nonhealing wounds: Emerging opportunities and challenges.Medicinal research reviews · 2021Review
- Adipose-Derived Stem Cells Secretome and Its Potential Application in "Stem Cell-Free Therapy".Biomolecules · 2021Review
Corrections and comments
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Authors and funding
24 authors at 6 institutions in 3 countries.
Funding
Abstract
Adipose-derived stem cells (ASCs) have become an important research model in regenerative medicine. However, there are controversies regarding the impact of prolonged cell culture on the ASCs phenotype and their differentiation potential. Hence, we studied 10 clinical ASCs replicates from plastic and oncological surgery patients, in six-passage FBS supplemented cultures. We quantified basic mesenchymal cell surface marker transcripts and the encoded proteins after each passage. In parallel, we investigated the differentiation potential of ASCs into chondrocytes, osteocytes and adipocytes. We further determined the effects of FBS supplementation and subsequent deprivation on the whole transcriptome by comprehensive mRNA and miRNA sequencing. Our results show that ASCs maintain differentiation potential and consistent profile of key mesenchymal markers, with apparent expression of distinct isoforms, in long-term cultures. No significant differences were observed between plastic and oncological surgery cohorts. ASCs in FBS supplemented primary cultures are almost committed to mesenchymal lineages as they express key epithelial-mesenchymal transition genes including early mesenchymal markers. Furthermore, combined mRNA/miRNA expression profiling strongly supports a modulatory role for the miR-30 family in the commitment process to mesenchymal lineages. Finally, we propose improvements to existing qPCR based assays that address alternative isoform expression of mesenchymal markers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.