ArticleStem cell research & therapy2018
Biphasic modulation of insulin signaling enables highly efficient hematopoietic differentiation from human pluripotent stem cells.
Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- 3D bioprinted human vascular organoid sheets promote functional ischemic repair and exhibit adaptive in vivo remodeling.BMC medicine · 2026Article
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- Induced pluripotent stem cell-based modeling of hemolytic anemia in patients with compound heterozygous KLF1 mutations reveals defective erythroid differentiation.Stem cell research & therapy · 2026Article
- Engineering of induced pluripotent stem cells for the efficient development of non-alloreactive, hypoimmunogenic CD8αβ CAR-T cells.Frontiers in immunology · 2026Article
- Chemically Defined, Efficient Megakaryocyte Production from Human Pluripotent Stem Cells.Cells · 2025Article
- ETV2 Overexpression Promotes Efficient Differentiation of Pluripotent Stem Cells to Endothelial Cells.Biotechnology and bioengineering · 2025Article
- A Comparative Study of Human Pluripotent Stem Cell-Derived Macrophages in Modeling Viral Infections.Viruses · 2024Article
- Human Pluripotent Stem Cells Derived Endothelial Cells Repair Choroidal Ischemia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Modeling antiviral response in the liver using human pluripotent stem cell-derived macrophages.Life medicine · 2024Article
- rAAV2-Mediated Restoration of GALC in Neural Stem Cells from Krabbe Patient-Derived iPSCs.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Engineered T cells from induced pluripotent stem cells: from research towards clinical implementation.Frontiers in immunology · 2023Review
- Single-cell atlas of multilineage cardiac organoids derived from human induced pluripotent stem cells.Life medicine · 2022Article
- Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate induction.Nature communications · 2022Article
- The Differentiation of Pluripotent Stem Cells towards Endothelial Progenitor Cells - Potential Application in Pulmonary Arterial Hypertension.International journal of stem cells · 2022Article
- Differential effects of macrophage subtypes on SARS-CoV-2 infection in a human pluripotent stem cell-derived model.Nature communications · 2022Article
- Efficient generation of endothelial cells from induced pluripotent stem cells derived from a patient with peripheral arterial disease.Cell and tissue research · 2022Article
- Insulin/IGF-1 signaling promotes immunosuppression via the STAT3 pathway: impact on the aging process and age-related diseases.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2021Review
- Transferrin improved the generation of cardiomyocyte from human pluripotent stem cells for myocardial infarction repair.Journal of molecular histology · 2021Article
- Overexpression of HOXA9 upregulates NF-κB signaling to promote human hematopoiesis and alter the hematopoietic differentiation potentials.Cell regeneration (London, England) · 2021Article
- Simulated Microgravity Potentiates Hematopoietic Differentiation of Human Pluripotent Stem Cells and Supports Formation of 3D Hematopoietic Cluster.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHematopoietic lineage cells derived from human pluripotent stem cells (hPSCs) hold great promise for the treatment of hematological diseases and providing sufficient cells for immune therapy. However, a simple, cost-effective method to generate large quantities of hematopoietic stem/progenitor cells (HSPCs) is not yet available.
methodsWe established a monolayer, chemically defined culture system to induce hematopoietic differentiation from hPSCs in 8 days.
resultsWe found that insulin-free medium allowed hPSCs to leave pluripotency promptly and preferably enter the vascular lineage. Addition of insulin during the later stage of differentiation was essential for the efficient induction of hemogenic endothelium and the emergence of large numbers of CD34
conclusionOur results demonstrated that proper manipulation of insulin-mTOR signaling can greatly facilitate HSPC formation. This finding can be further exploited to formulate cost-effective differentiation medium to generate large quantities of cells of desired blood lineages for regenerative medicine.
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