ArticleStem cell research & therapy2018
In vitro osteoblastic differentiation of mesenchymal stem cells generates cell layers with distinct properties.
Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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91 citing papers in PubMed, 172 citations in OpenAlex.
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- Triazine-Trione Thermosets with High Processability for Scaffold Applications in Bone Tissue Engineering.Advanced healthcare materials · 2026Article
- A workflow integrating organ-on-chip culture and correlative 3D light and electron microscopy for microtissue analysis.Scientific reports · 2025Article
- Synergistic Advances in Additive Manufacturing and Surface Engineering for Polymeric Biomedical Devices.ACS polymers Au · 2025Review
- Deep-freezing and decontamination strategy for a large autologous bone graft with presentation of the osteogenic potential of resident osteoblasts.Stem cell research & therapy · 2025Article
- Hydrostatic Pressure Induces Osteogenic Differentiation of Single Stem Cells in 3D Viscoelastic Microgels.Small science · 2025Article
- Calcium oscillations in mesenchymal stem cells, a control on cell cycle progression to influence cell fate towards proliferation or differentiation?Stem cell research & therapy · 2025Article
- Development of optimized differentiation media for murine bone marrow multipotent mesenchymal stromal cells.Histochemistry and cell biology · 2025Article
- Bioinspired 3D Bone Model: Mimicking the Cortical-Spongy Bone Architecture and Biology for Enhanced Physiological Representation of Bone.ACS biomaterials science & engineering · 2025Article
- Tunable Release of Ions from Graphene Oxide Laminates for Sustained Antibacterial Activity in a Biomimetic Environment.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights fromMaterials today. Bio · 2025Article
- Biodentine Stimulates Calcium-Dependent Osteogenic Differentiation of Mesenchymal Stromal Cells from Periapical Lesions.International journal of molecular sciences · 2025Article
- Guidance on the assessment of the functionality of biomaterials for periodontal tissue regeneration: Methodologies and testing procedures.Dental materials : official publication of the Academy of Dental Materials · 2025Review
- Stem cell therapy for regenerating periodontal bony defects: A narrative review.Journal of advanced periodontology & implant dentistry · 2025Review
- Synergistic potential of stem cells and microfluidics in regenerative medicine.Molecular and cellular biochemistry · 2025Review
- Early osteogenic differentiation of human dental stem cells by gelatin/calcium phosphate- Punica granatum nanocomposite scaffold.BMC biotechnology · 2025Article
- Structure-function integrated biodegradable Mg/polymer composites: Design, manufacturing, properties, and biomedical applications.Bioactive materials · 2024Review
- Image-based discrimination of the early stages of mesenchymal stem cell differentiation.Molecular biology of the cell · 2024Article
31 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
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Abstract
backgroundDifferentiation of mesenchymal stem cells to osteoblasts is widely performed in research laboratories. Classical tests to prove this differentiation employ procedures such as cell fixation, cell lysis or cell scraping. Very few studies report gentle dissociation of mesenchymal stem cells undergoing an osteodifferentiation process. Here we used this technique to reveal the presence of several cell layers during osteogenesis and to study their different properties.
methodsThrough the sequential enzymatic detachment of the cells, we confirm the presence of several layers of differentiated cells and we compare them in terms of enzymatic sensitivity for dissociation, expression of cluster of differentiation, cytosolic calcium oscillations and osteogenic potential. Adipogenic and neurogenic differentiations were also performed in order to compare the cell layers.
resultsThe cells undergoing differentiation formed one layer in the neurogenic differentiation, two layers in the adipogenic differentiation and at least four layers in the osteogenic differentiation. In the latter, the upper layers, maintained by a collagen I extracellular matrix, can be dissociated using collagenase I, while the remaining lowest layer, attached to the bottom of the dish, is sensitive only to trypsin-versene. The action of collagenase I is more efficient before the mineralization of the extracellular matrix. The collagenase-sensitive and trypsin-sensitive layers differ in their cluster of differentiation expression. The dissociation of the cells on day 15 reveals that cells could resume their growth (increase in cell number) and rapidly differentiate again in osteoblasts, in 2 weeks (instead of 4 weeks). Cells from the upper layers displayed a higher mineralization.
conclusionsMSCs undergoing osteogenic differentiation form several layers with distinct osteogenic properties. This could allow the investigators to use upper layers to rapidly produce differentiated osteoblasts and the lowest layer to continue growth and differentiation until an ulterior dissociation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.