Evidence map›Paper›PMID 30053878›Full record

ArticleMolecular cancer2018

Knockdown of long non-coding RNA NEAT1 inhibits glioma cell migration and invasion via modulation of SOX2 targeted by miR-132.

Ke Zhou, Chi Zhang, Hui Yao, Xuewen Zhang, Youxin Zhou, Yanjun Che, Yulun Huang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
132citing papers in PubMed, 1 pooled it
11.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

132 citing papers in PubMed, 1 synthesis or guideline pooled it, 224 citations in OpenAlex.

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72 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ke ZhouDepartment of Neurosurgery, the First Affiliated Hospital of Soochow University, No.188, Shizi Street, Suzhou, 215007, Jiangsu, China.
Chi ZhangDepartment of Neurosurgery, the First Affiliated Hospital of Soochow University, No.188, Shizi Street, Suzhou, 215007, Jiangsu, China.
Hui YaoDepartment of Neurosurgery, the First Affiliated Hospital of Soochow University, No.188, Shizi Street, Suzhou, 215007, Jiangsu, China.
Xuewen ZhangDepartment of Neurosurgery, the First Affiliated Hospital of Soochow University, No.188, Shizi Street, Suzhou, 215007, Jiangsu, China.
Youxin ZhouDepartment of Neurosurgery, the First Affiliated Hospital of Soochow University, No.188, Shizi Street, Suzhou, 215007, Jiangsu, China.
Yanjun CheDepartment of Neurosurgery, Jingjiang People's Hospital, No. 28 East Zhongzhou Road, Taizhou, 214500, Jiangsu, China. hanxiuzhen1119@126.com.
Yulun HuangDepartment of Neurosurgery, the First Affiliated Hospital of Soochow University, No.188, Shizi Street, Suzhou, 215007, Jiangsu, China. huangyulun@suda.edu.cn.
First Affiliated Hospital of Soochow University · CNSoochow University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundA better understanding of the molecular mechanism involving lncRNA-miRNA-mRNA network underlying glioma genesis is beneficial to the treatment of glioma. This study was designed to investigate the role of lncRNA NEAT1, miR-132 and SOX2 interaction in glioma.

methodsMicroarray analysis was conducted to identify the differentially expressed lncRNAs in glioma tissues. The expression levels of NEAT1, miR-132 and SOX2 were determined by qRT-PCR and western blot. Proliferation of glioma cells was detected by MTT assay, while migration and invasion were determined by transwell assay. The target relationships were predicted by miRcode algorithm, and confirmed by dual luciferase reporter gene assay.

resultsNEAT1 was up-regulated in glioma. Knockdown of NEAT1 inhibited glioma cells' viability, migration and invasion. MiR-132 was down-regulated while SOX2 was up-regulated in glioma cells. NEAT1 negatively regulated the expression of miR-132 in glioma while miR-132 targeted SOX2 to down-regulate its expression.

conclusionNEAT1 promoted glioma development by promoting SOX2 expression through suppressing miR-132.

Indexed as

Brain NeoplasmsCell Line, TumorCell MovementGene Expression ProfilingGene Expression Regulation, NeoplasticGene Knockdown TechniquesGliomaHumansMicroRNAsNeoplasm InvasivenessNeoplasm StagingOligonucleotide Array Sequence AnalysisRNA, Long NoncodingSOXB1 Transcription FactorsUp-RegulationMicroRNAsMIRN132 microRNA, humanNEAT1 long non-coding RNA, humanRNA, Long NoncodingSOX2 protein, humanSOXB1 Transcription FactorsGliomaInvasionmicroRNA-132MigrationNEAT1SOX2

Identifiers

PMID30053878
PMCPMC6064054
OpenAlexW2883978878

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.