ArticleBMC complementary and alternative medicine2018
Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide).
Article in BMC complementary and alternative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Human Histatin 5 Exerts Anti-Trypanosomal Activity AgainstInternational journal of molecular sciences · 2026Article
- Antiamoeba and Antitrypanosoma Activity of the MexicanPathogens (Basel, Switzerland) · 2026Article
- Trypanocidal Activity of Crude Extracts fromJournal of fungi (Basel, Switzerland) · 2026Article
- Plant-Derived Terpenes as Emerging Therapeutics Against Schistosomiasis.International journal of molecular sciences · 2026Review
- Parasiticidal Activity of Citral Against Enteromyxum leei (myxozoa: myxosporea) in Olive Flounder (Paralichthys olivaceus).Acta parasitologica · 2025Article
- Mechanisms and Applications of Citral's Antimicrobial Properties in Food Preservation and Pharmaceuticals Formulations.Antibiotics (Basel, Switzerland) · 2023Review
- In Vitro Activity of Essential Oils Distilled from Colombian Plants againstMolecules (Basel, Switzerland) · 2022Article
- Evaluation of the In Vitro Antiparasitic Effect of the Essential Oil ofMolecules (Basel, Switzerland) · 2022Article
- A Potential New Source of Therapeutic Agents for the Treatment of Mucocutaneous Leishmaniasis: The Essential Oil ofMolecules (Basel, Switzerland) · 2022Article
- A terpenoid-rich extract fromHeliyon · 2022Article
- Essential oils from Melia azedarach L. (Meliaceae) leaves: chemical variability upon environmental factors.Journal of natural medicines · 2022Article
- Caryophyllene Oxide Induces Ferritinophagy by Regulating the NCOA4/FTH1/LC3 Pathway in Hepatocellular Carcinoma.Frontiers in pharmacology · 2022Article
- Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes fromAntioxidants (Basel, Switzerland) · 2021Article
- Cytotoxicity of Essential OilMolecules (Basel, Switzerland) · 2021Article
- Immunomodulatory, trypanocide, and antioxidant properties of essential oil fractions of Lippia alba (Verbenaceae).BMC complementary medicine and therapies · 2021Article
- Joining forces: Leveraging novel combination therapies to combat infections with eukaryotic pathogens.PLoS pathogens · 2020Article
- Trypanocidal Essential Oils: A Review.Molecules (Basel, Switzerland) · 2020Review
- In Vitro and In Vivo Inhibitory Activity of Limonene against Different Isolates ofJournal of fungi (Basel, Switzerland) · 2020Article
- First Report on Yield and Chemical Composition of Essential Oil Extracted fromMolecules (Basel, Switzerland) · 2020Article
- Chemical Composition, Antimicrobial and Antiparasitic Screening of the Essential Oil fromMolecules (Basel, Switzerland) · 2019Article
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4 authors.
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Abstract
backgroundChagas Disease caused by Trypanosoma cruzi infection, is one of the most important neglected tropical diseases (NTD), without an effective therapy for the successful parasite eradication or for the blocking of the disease's progression, in its advanced stages. Due to their low toxicity, wide pharmacologic spectrum, and potential synergies, medicinal plants as Lippia alba, offer a promising reserve of bioactive molecules. The principal goal of this work is to characterize the inhibitory properties and cellular effects of the Citral and Carvone L. alba chemotype essential oils (EOs) and their main bioactive terpenes (and the synergies among them) on T. cruzi forms.
methodsTwelve L. alba EOs, produced under diverse environmental conditions, were extracted by microwave assisted hydrodistillation, and chemically characterized using gas chromatography coupled mass spectrometry. Trypanocidal activity and cytotoxicity were determined for each oil, and their major compounds, on epimastigotes (Epi), trypomastigotes (Tryp), amastigotes (Amas), and Vero cells. Pharmacologic interactions were defined by a matrix of combinations among the most trypanocidal terpenes (limonene, carvone; citral and caryophyllene oxide). The treated cell phenotype was assessed by fluorescent and optic microscopy, flow cytometry, and DNA electrophoresis assays.
resultsThe L. alba EOs displayed significant differences in their chemical composition and trypanocidal performance (p = 0.0001). Citral chemotype oils were more trypanocidal than Carvone EOs, with Inhibitory Concentration 50 (IC
conclusionsEOs extracted from L. alba Citral chemotype demonstrated significant trypanocidal activity on the three forms of T. cruzi studied, and their composition and trypanocidal performance were influenced by production parameters. Citral, caryophyllene oxide, and limonene showed a possible induction of an apoptotic-like phenotype. The best selective anti-T. cruzi activity was achieved by limonene, the effects of which were also synergic with citral, caryophyllene oxide and benznidazole.
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