Evidence map›Paper›PMID 30053393›Full record

ArticleMolecular and biochemical parasitology2018

Expression of full-length Plasmodium falciparum P48/45 in P. berghei blood stages: A method to express and evaluate vaccine antigens.

Ahmad Syibli Othman, Jing-Wen Lin, Blandine M Franke-Fayard, Hans Kroeze, Fiona J A van Pul, Séverine Chevalley-Maurel, Jai Ramesar, Catherin Marin-Mogollon, Matthijs M Jore, Merribeth J Morin and 6 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and biochemical parasitology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Science translational medicine · 2021
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 4 countries.

Ahmad Syibli OthmanLeiden Malaria Research Group, Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands; Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Terengganu, Malaysia.
Jing-Wen LinDivision of Pediatric Infectious Diseases, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University and Collaboration Innovation Center, Chengdu, China.
Blandine M Franke-FayardLeiden Malaria Research Group, Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Hans KroezeLeiden Malaria Research Group, Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Fiona J A van PulLeiden Malaria Research Group, Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Séverine Chevalley-MaurelLeiden Malaria Research Group, Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Jai RamesarLeiden Malaria Research Group, Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Catherin Marin-MogollonLeiden Malaria Research Group, Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Matthijs M JoreDepartment of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
Merribeth J MorinPATH's Malaria Vaccine Initiative, Washington DC, USA.
Carole A LongLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Rockville, Maryland, USA.
Robert SauerweinDepartment of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
Ashley BirkettPATH's Malaria Vaccine Initiative, Washington DC, USA.
Kazutoyo MiuraLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Rockville, Maryland, USA.
Chris J JanseLeiden Malaria Research Group, Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
Shahid M KhanLeiden Malaria Research Group, Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands. Electronic address: S.M.Khan@lumc.nl.
Leiden University Medical Center · NLNational Institute of Allergy and Infectious Diseases · USRadboud University Nijmegen · NLU.S. President's Malaria Initiative · USSichuan University · CN

Funding

Host Immune Responses to Antigens of Malaria ParasitesZIAAI001020 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI LONG, CAROLE · 2009 to 2025
$13.6M
Intramural NIH HHS Z99 AI999999
6 · The paper itself

Abstract

The transmission-blocking vaccine candidate Pfs48/45 from the human malaria parasite Plasmodium falciparum is known to be difficult to express in heterologous systems, either as full-length protein or as correctly folded protein fragments that retain conformational epitopes. In this study we express full-length Pfs48/45 in the rodent parasite P. berghei. Pfs48/45 is expressed as a transgene under control of the strong P. berghei schizont-specific msp1 gene promoter (Pfs48/45@PbMSP1). Pfs48/45@PbMSP1 schizont-infected red blood cells produced full-length Pfs48/45 and the structural integrity of Pfs48/45 was confirmed using a panel of conformation-specific monoclonal antibodies that bind to different Pfs48/45 epitopes. Sera from mice immunized with transgenic Pfs48/45@PbMSP1 schizonts showed strong transmission-reducing activity in mosquitoes infected with P. falciparum using standard membrane feeding. These results demonstrate that transgenic rodent malaria parasites expressing human malaria antigens may be used as means to evaluate immunogenicity and functionality of difficult to express malaria vaccine candidate antigens.

Indexed as

AnimalsAntigens, ProtozoanDisease Models, AnimalDisease Transmission, InfectiousGene ExpressionMalariaMalaria VaccinesMembrane ProteinsMicePlasmodium bergheiPlasmodium falciparumPromoter Regions, GeneticRecombinant ProteinsTransgenesAntigens, ProtozoanMalaria VaccinesMembrane ProteinsRecombinant ProteinsImmunizationPfs48/45PlasmodiumStandard membrane feedingTransgene-expressionTransmission blocking

Identifiers

PMID30053393
PMCPMC11040549
OpenAlexW2883976421

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.