ArticleProtein science : a publication of the Protein Society2018
A protein-protein host-guest complex: Thermostable ferritin encapsulating positively supercharged green fluorescent protein.
Article in Protein science : a publication of the Protein Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Charge engineering controls cooperative assembly and loading in protein host-guest complexes.Journal of materials chemistry. B · 2025Article
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- Magnetizing Biotech-Advances in (In Vivo) Magnetic Enzyme Immobilization.Engineering in life sciences · 2025Review
- Emerging Landscape of Supercharged Proteins and Peptides for Drug Delivery.ACS pharmacology & translational science · 2024Review
- Molecular Engineering ofMolecules (Basel, Switzerland) · 2023Article
- Assembly of Protein Cages for Drug Delivery.Pharmaceutics · 2022Review
- Article
- Nanoencapsulation as a General Solution for Lyophilization of Labile Substrates.Pharmaceutics · 2021Article
- Artificial Protein Cage Delivers Active Protein Cargos to the Cell Interior.Biomacromolecules · 2021Article
- Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells.Scientific reports · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We characterize the encapsulation of supercharged green fluorescent protein, GFP(+36), by thermophilic ferritin from Archaeoglobus fulgidus (AfFtn). The AfFtn-GFP(+36) assembly is rapid, nearly stoichiometric, and robust. Using a more stably assembled mutant AfFtn, we show that encapsulation can occur in the presence of mostly assembled cages, in addition to encapsulation starting from AfFtn individual subunits. Assembly and encapsulation do not occur with non-supercharged GFP or the alternately supercharged GFP(-30), highlighting the role of complementary electrostatic interactions between the cargo and AfFtn cage interior. We also present a method for verifying protein-protein encapsulation, using nickel nitrilotriacetic acid agarose resin. AfFtn-supercharged protein host-guest complexes could find applications in enzyme studies, protein separations, and in vivo protein stabilization and targeted delivery.
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Registered trials
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