Evidence map›Paper›PMID 30051426›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Exploiting Phage Display for Development of Novel Cellular Targeting Strategies.

William Marsh, Amanda Witten, Sarah E Stabenfeldt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

William MarshSchool of Biological and Health Systems Engineering, Ira A. Fulton Schools of Engineering, Arizona State University, Tempe, AZ, USA.
Amanda WittenSchool of Biological and Health Systems Engineering, Ira A. Fulton Schools of Engineering, Arizona State University, Tempe, AZ, USA.
Sarah E StabenfeldtSchool of Biological and Health Systems Engineering, Ira A. Fulton Schools of Engineering, Arizona State University, Tempe, AZ, USA. sarah.stabenfeldt@asu.edu.

Funding

Detecting and Treating Traumatic Brain Injury Pathology Progression from the Inside-OutDP2HD084067 · NICHD · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI STABENFELDT, SARAH E · 2014 to 2018
$2.8M
NICHD NIH HHS DP2 HD084067
6 · The paper itself

Abstract

Targeting strategies for drug delivery applications rely on targeting moieties (i.e., peptide, antibody) specific to the desired cell surface receptor or protein of interest. However, current targeting strategies are limited to previously identified epitopes/ligand pairs. The field of phage display opens up the targeting moiety options whereby new epitope/ligand pairs may be discovered through well-designed biopanning assays for the target cell population of interest. Here, we provide a detailed protocol to perform phage biopanning assays on adherent cell cultures. The methods described here may be modified to user-specific targeting interests.

Indexed as

BacteriophagesCell AdhesionCell LineCell Surface Display TechniquesEnzyme-Linked Immunosorbent AssayHumansSingle-Chain AntibodiesSingle-Chain AntibodiesAstrocytesDomain antibody (dAb)Phage displaySingle chain antibody fragment (scFv)

Identifiers

PMID30051426
PMCPMC6205264

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.