Evidence map›Paper›PMID 30051305›Full record

ArticleMolecular and cellular biochemistry2019

Protein kinases orchestrate cell cycle regulators in differentiating BeWo choriocarcinoma cells.

M Naveen Kumar, R L Babu, Rajeshwari H Patil, K M Kiran Kumar, Shubha M Hegde, Rashmi Nagesh, K Kavya, Govindarajan T Ramesh, S Chidananda Sharma, C Srinivas

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Article in Molecular and cellular biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

M Naveen KumarDepartment of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560 056, Karnataka, India.
R L BabuDepartment of Bioinformatics and Biotechnology, Akkamahadevi Women's University, Jnanashakthi Campus, Vijayapura, 586 108, Karnataka, India.
Rajeshwari H PatilDepartment of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560 056, Karnataka, India.
K M Kiran KumarDepartment of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560 056, Karnataka, India.
Shubha M HegdeDepartment of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560 056, Karnataka, India.
Rashmi NageshDepartment of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560 056, Karnataka, India.
K KavyaDepartment of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560 056, Karnataka, India.
Govindarajan T RameshDepartment of Biology and Center for Biotechnology and Biomedical Sciences, Norfolk State University, Norfolk, VA, USA.
S Chidananda SharmaDepartment of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560 056, Karnataka, India.
C SrinivasDepartment of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560 056, Karnataka, India. csrinivas@bub.ernet.in.
Bangalore University · INKarnataka State Women's University · INNorfolk State University · US

Funding

Department of Science and Technology-Promotion of University Research and Scientific Excellence (DST-PURSE) [SR/59/Z-23/2010/38(c)]University Grant Commission-Centre with Potential for Excellence in Particular Area (UGC-CPEPA) [8-2/2008(NS/PE)]
6 · The paper itself

Abstract

Choriocarcinoma, a trophoblastic neoplasia, occurs in women as an incidence of abnormal pregnancy. BeWo choriocarcinoma cells derived from the abnormal placentation are a suitable model system to study the factors associated with differentiation, invasion and other cellular events as an alternative to clinical samples. Many protein kinases orchestrate the complex events of cell cycle and in case of malignancy such regulators are found to be mutated. In the present study, BeWo cells treated with forskolin (Fo) and phorbol 12-myristate 13-acetate (PMA) were used to study the role of PKA (protein kinase A) and PKC (protein kinase C), respectively, on the expression pattern of differentiation-related genes, membrane markers, PKC isoforms and cell cycle regulators. The effect of Fo and PMA on the cell proliferation was assessed. Progressive induction of alkaline phosphatase level and formation of multinucleated differentiated cells were observed in the cells treated with Fo. Exposure of cells to Fo and PMA induced the mRNA transcripts of α-hCG, β-hCG and endoglin and down-regulates E-cadherin at mRNA and protein levels. Synergistic levels of both up- and down-regulated genes/proteins were observed when cells were treated with the combination of Fo and PMA. The mRNA levels of cyclin D1, cyclin E1, p21, Rb, p53, caspase-3 and caspase-8 decreased gradually during differentiation. Fo significantly inhibited the protein levels of PCNA, Rb, PKC-α and PMA stimulated mRNA expression of PKC-ε and PKC-δ. Further, failure in the activation of essential components of the cell cycle machinery caused G2/M phase arrest in differentiating BeWo cells.

Indexed as

Cell CycleCell DifferentiationCell Cycle ProteinsCell ProliferationChoriocarcinomaHumansProtein KinasesTumor Cells, CulturedCell Cycle ProteinsProtein KinasesBeWo choriocarcinoma cellsCell cycleDifferentiationForskolinPhorbol estersProtein kinases

Identifiers

PMID30051305
OpenAlexW2884198698

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.