ArticleStem cell investigation2018
Temporary storage solution for adipose derived mesenchymal stem cells.
Article in Stem cell investigation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 11 citations in OpenAlex.
- Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants.Regenerative therapy · 2023Article
- Effects of carrier solutions on the viability and efficacy of canine adipose-derived mesenchymal stem cells.BMC veterinary research · 2022Article
- Article
- Adipose Tissue Stem Cells for Therapy: An Update on the Progress of Isolation, Culture, Storage, and Clinical Application.Journal of clinical medicine · 2019Review
- Effects of Normothermic Machine Perfusion Conditions on Mesenchymal Stromal Cells.Frontiers in immunology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe have developed a simple lipoaspirate washing method using a coffee filter to eliminate liposuction noxious material before isolating adipose tissue derived mesenchymal stem cells (AT-MSCs), and used them in clinical trials. Before administration to patients, MSCs are usually suspended in physiologic saline. However, MSCs only survive for a limited time in physiologic saline. Therefore, alternative solution that can preserve MSC survival will be beneficial. Therefore, the purpose of this study was to compare the use of physiologic saline and Dulbecco's modified Eagle's Medium (DMEM) as temporary storage solution for our AT-MSCs.
methodsWe did viability assessments of AT MSCs after 0, 3, 6, 24, 48, 72, and 96 hours suspended in physiologic saline compared to DMEM, and stored at 4 ℃. Further proliferation capacities of the cells after various suspension times were assessed. All viability and proliferation capacity assessments were done in four replications. Differences between the various suspension time in terms of viability and proliferation capacity were compared and tested by ANOVA or Kruskal-Wallis test.
resultsViability was >70% after 48 hours in physiologic saline and 24 hours in DMEM, which showed that physiologic saline was superior compared to DMEM. Increase in PDT began to be significant compared to initial PDT after 24 hours in both physiologic saline, and DMEM.
conclusionsFor our AT-MSCs, physiologic saline was superior to DMEM, and storage should not exceed 24 hours.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.