ArticleMalaria journal2018
In vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte-adapted P. knowlesi A1-H.1 strain.
Article in Malaria journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.
- High-throughput screening and meta-analysis for lead compounds in antimalarial drug discovery.Malaria journal · 2025Pooled it
- Development of an Experimental Human Blood-Stage Model for Studying Plasmodium knowlesi.The Journal of infectious diseases · 2026Article
- Impact ofCurrent research in parasitology & vector-borne diseases · 2026Article
- A novel micronemal protein MP38 is involved in the invasion of merozoites into erythrocytes.mBio · 2025Article
- Red blood cell signaling is functionally conserved iniScience · 2024Article
- Characterization of merozoite-specific thrombospondin-related anonymous protein (MTRAP) inFrontiers in cellular and infection microbiology · 2024Article
- Review
- Inter-Population Genetic Diversity and Clustering ofTropical medicine and infectious disease · 2023Article
- The Impact of Geographical Variation inTropical medicine and infectious disease · 2023Article
- High opsonic phagocytosis activity and growth inhibition of merozoites are associated with RON4 antibody levels and protect against febrile malaria in Ghanaian children.Frontiers in immunology · 2023Article
- Merozoite surface protein 1 paralog is involved in the human erythrocyte invasion of a zoonotic malaria,Frontiers in cellular and infection microbiology · 2023Article
- Plasmodium knowlesi: the game changer for malaria eradication.Malaria journal · 2022Review
- Using Plasmodium knowlesi as a model for screening Plasmodium vivax blood-stage malaria vaccine targets reveals new candidates.PLoS pathogens · 2021Article
- Identification of Reticulocyte Binding Domain ofFrontiers in cellular and infection microbiology · 2021Article
- Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi.PLoS neglected tropical diseases · 2020Article
- Molecular characterization and immune protection of an AN1-like zinc finger protein of Eimeria tenella.Parasitology research · 2020Article
- Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1.Parasite (Paris, France) · 2020Article
- Reverse immunodynamics: a new method for identifying targets of protective immunity.Scientific reports · 2019Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundThe rapid process of malaria erythrocyte invasion involves ligand-receptor interactions. Inducing antibodies against specific ligands or receptors that abrogate the invasion process is a key challenge for blood stage vaccine development. However, few candidates were reported and remain to be validated for the discovery of new vaccine candidates in Plasmodium knowlesi.
methodsIn order to investigate the efficacy of pre-clinical vaccine candidates in P. knowlesi-infected human cases, this study describes an in vitro invasion inhibition assay, using a P. knowlesi strain adapted to in vitro growth in human erythrocytes, PkA1-H.1. Recombinant proteins of P. knowlesi Duffy binding protein alpha (PkDBPα) and apical membrane antigen 1 (PkAMA1) were produced in Escherichia coli system and rabbit antibodies were generated from immune animals.
resultsPkDBPα and PkAMA1 recombinant proteins were expressed as insoluble and produced as a functional refolded form for this study. Antibodies against PkDBPα and PkAMA1 specifically recognized recombinant proteins and native parasite proteins in schizont-stage parasites on the merozoite organelles. Single and combination of anti-PkDBPα and anti-PkAMA1 antibodies elicited strong growth inhibitory effects on the parasite in concentration-dependent manner. Meanwhile, IgG prevalence of PkDBPα and PkAMA1 were observed in 13.0 and 46.7% in human clinical patients, respectively.
conclusionThese data provide support for the validation of in vitro growth inhibition assay using antibodies of DBPα and AMA1 in human-adapted P. knowlesi parasite PkA1-H.1 strain.
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