ArticleGenes & genomics2018
Molecular cloning and characterization of a novel salt-specific responsive WRKY transcription factor gene IlWRKY2 from the halophyte Iris lactea var. chinensis.
Article in Genes & genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Article
- Article
- Physiological and Transcriptional Responses ofInternational journal of molecular sciences · 2023Article
- Isolation of oligostilbenes fromRSC advances · 2022Article
- Molecular Pathways of WRKY Genes in Regulating Plant Salinity Tolerance.International journal of molecular sciences · 2022Review
- Characterization and phylogenetic analysis of the chloroplast genome ofMitochondrial DNA. Part B, Resources · 2021Article
- Article
- Article
- The Overexpression of a Transcription Factor GeneFrontiers in plant science · 2019Article
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Authors and funding
6 authors.
Funding
Abstract
Iris lactea var. chinensis is a perennial herbaceous halophyte with high salt tolerance and ornamental value. Previous RNA sequencing analysis revealed a transcription factor gene IlWRKY2 expression was upregulated by salt stress. To obtain the full-length sequence, the basic characteristics of IlWRKY2 and its expression pattern under salt stress. Full-length cDNA of IlWRKY2 was cloned by 3'/5' RACE based on the intermediate sequence obtained by RNA sequencing analysis. Structure analysis of IlWRKY2 were performed by Compute pI/MW tool, PSIPRED and SWISS-MODEL analysis. Sequence analysis of IlWRKY2 were performed by BLAST program, DNAman software, MEGA software and MEME program. IlWRKY2 expression pattern was analyzed by quantitative real-time polymerase chain reaction. The open reading frame of IlWRKY2 is 1338 bp in length, which encodes a protein of 446 amino acids. Amino acid sequence analysis revealed that the IlWRKY2 contains one WRKY domains with a zinc finger motif C-X
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