ArticleJournal of industrial microbiology & biotechnology2018
Engineering Escherichia coli to increase triacetic acid lactone (TAL) production using an optimized TAL sensor-reporter system.
Article in Journal of industrial microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- A highly active Burkholderia polyketoacyl-CoA thiolase for production of triacetic acid lactone.Nature communications · 2025Article
- Production of triacetic acid lactone from oleic acid by engineering the yeast Candida viswanathii.Microbial cell factories · 2025Article
- A heterologous expression platform inChemical science · 2023Article
- Advanced one-pot deconstruction and valorization of lignocellulosic biomass into triacetic acid lactone using Rhodosporidium toruloides.Microbial cell factories · 2022Article
- Metabolic engineering of oleaginous yeast Rhodotorula toruloides for overproduction of triacetic acid lactone.Biotechnology and bioengineering · 2022Article
- Protein engineering for natural product biosynthesis and synthetic biology applications.Protein engineering, design & selection : PEDS · 2021Review
- Current Challenges and Opportunities in Non-native Chemical Production by Engineered Yeasts.Frontiers in bioengineering and biotechnology · 2020Review
- Biosensor-guided improvements in salicylate production by recombinant Escherichia coli.Microbial cell factories · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Triacetic acid lactone (TAL) (4-hydroxy-6-methyl-2-pyrone) can be upgraded into a variety of higher-value products, and has potential to be developed into a renewable platform chemical through metabolic engineering. We previously developed an endogenous TAL sensor based on the regulatory protein AraC, and applied it to screen 2-pyrone synthase (2-PS) variant libraries in E. coli, resulting in the identification of variants conferring up to 20-fold improved TAL production in liquid culture. In this study, the sensor-reporter system was further optimized and used to further improve TAL production from recombinant E. coli, this time by screening a genomic overexpression library. We identified new and unpredictable gene targets (betT, ompN, and pykA), whose plasmid-based expression improved TAL yield (mg/L/OD
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.