ArticleInvestigative ophthalmology & visual science2018
Hyaluronan Regulates Eyelid and Meibomian Gland Morphogenesis.
Article in Investigative ophthalmology & visual science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Molecular Target Discovery and Systemic Mechanism Analysis of Teriflunomide for Dry Eye Disease.Current issues in molecular biology · 2026Article
- Comparative Analysis of Age-Associated Changes in Meibum Composition, Distribution, and Function in Mice With Altered Hyaluronan Expression.Investigative ophthalmology & visual science · 2025Article
- The Yin and Yang of non-immune and immune responses in meibomian gland dysfunction.The ocular surface · 2024Article
- Age related changes in hyaluronan expression leads to Meibomian gland dysfunction.Matrix biology : journal of the International Society for Matrix Biology · 2023Article
- ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing.Biomolecules · 2023Article
- Toxicological effects of ocular acrolein exposure to eyelids in rabbits in vivo.Experimental eye research · 2023Article
- Characterization of the Molecular Weight of Hyaluronan in Eye Products Using a Novel Method of Size Exclusion High-Pressure Liquid Chromatography.Translational vision science & technology · 2023Article
- Primary cilia control cellular patterning of Meibomian glands during morphogenesis but not lipid composition.Communications biology · 2023Article
- Hyaluronan Modulates the Biomechanical Properties of the Cornea.Investigative ophthalmology & visual science · 2022Article
- The Eyelid Meibomian Gland Deficiency in Fucosyltransferase 1 Knockout Mice.International journal of molecular sciences · 2022Article
- Hyaluronan supports the limbal stem cell phenotype during ex vivo culture.Stem cell research & therapy · 2022Article
- Expression of extracellular matrix components in the meibomian gland.Frontiers in medicine · 2022Article
- Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice.International journal of molecular sciences · 2021Article
- Meibomian Gland Dysfunction: What Have Animal Models Taught Us?International journal of molecular sciences · 2020Review
- Epithelial Cell Migration and Proliferation Patterns During Initial Wound Closure in Normal Mice and an Experimental Model of Limbal Stem Cell Deficiency.Investigative ophthalmology & visual science · 2020Article
- Distribution and Function of Glycosaminoglycans and Proteoglycans in the Development, Homeostasis and Pathology of the Ocular Surface.Frontiers in cell and developmental biology · 2020Review
- Hyaluronan Derived From the Limbus is a Key Regulator of Corneal Lymphangiogenesis.Investigative ophthalmology & visual science · 2019Article
- Meibomian gland development: Where, when and how?Differentiation; research in biological diversityReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Purpose: The Meibomian gland (MG) produces the lipid layer of the tear film, and changes to the MG that lead to a decrease or alteration in lipid quality/content may lead to MG dysfunction, a major cause of evaporative dry eye disease with prevalence ranging from 39% to 50%. Little is known about the developmental cues that regulate MG morphogenesis and homeostasis. Our study investigates the role of hyaluronan (HA), a major extracellular matrix component, in eyelid formation and MG development and function. Methods: Hyaluronan synthase (Has) knockout mice were used to determine the role of HA in the eyelid and MG. Eyelids were obtained during different developmental stages and MG morphology was analyzed. Tet-off H2B-GFP/K5tTA mice and 5-ethynyl-2'-deoxyurdine (EdU) incorporation were used to determine the role of HA in maintaining slow-cycling and proliferating cells within the MG, respectively. Data were confirmed using an in vitro proliferation assay, differentiation assay and spheroid cultures. Results: Has knockout mice present precocious MG development, and adult mice present MG hyperplasia and dysmorphic MGs and eyelids, with hyperplastic growths arising from the palpebral conjunctiva. Our data show that a highly organized HA network encompasses the MG, and basal cells are embedded within this HA matrix, which supports the proliferating cells. Spheroid cultures showed that HA promotes acini formation. Conclusions: HA plays an important role in MG and eyelid development. Our findings suggest that Has knockout mice have abnormal HA synthesis, which in turn leads to precocious and exacerbated MG morphogenesis culminating in dysmorphic eyelids and MGs.
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