ArticleScientific reports2018
Metabolomic analysis of mouse prefrontal cortex reveals upregulated analytes during wakefulness compared to sleep.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Mapping Brain Spatial Metabolic Atlas Reveals Anesthesia-Induced Region-Specific Alterations.Neuroscience bulletin · 2026Article
- Exploring Causal Associations Between Plasma Metabolites and Sciatica: A Two-Sample Mendelian Randomization Analysis.Journal of pain research · 2026Article
- Sampling Probes Microfabricated from Parylene‑C for In Vivo Neurochemical Monitoring.ACS measurement science au · 2025Article
- Improving the Detection of Analyte Degeneracies in Untargeted Liquid Chromatography-Tandem Mass Spectrometry Data.Analytical chemistry · 2025Article
- In-Depth Chemical Analysis of the Brain Extracellular Space UsingAnalytical chemistry · 2024Article
- Altered Metabolism during the Dark Period in Drosophila Short Sleep Mutants.Journal of proteome research · 2024Article
- Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery from sleep deprivation.Cell reports · 2024Article
- Targeted metabolomics-based understanding of the sleep disturbances in drug-naïve patients with schizophrenia.BMC psychiatry · 2024Article
- Metabolomic profiles of sleep-disordered breathing are associated with hypertension and diabetes mellitus development.Nature communications · 2024Article
- Integrated gut microbiome and metabolomic analyses elucidate the therapeutic mechanisms of Suanzaoren decoction in insomnia and depression models.Frontiers in neuroscience · 2024Article
- Assessing the associations of 1,400 blood metabolites with major depressive disorder: a Mendelian randomization study.Frontiers in psychiatry · 2024Article
- Discrimination exposure impacts unhealthy processing of food cues: crosstalk between the brain and gut.Nature. Mental health · 2023Article
- Neural consequences of chronic sleep disruption.Trends in neurosciences · 2022Review
- Vascular Endothelial Growth Factor May Be Involved in the Behavioral Changes of Progeny Rats after Exposure to Ceftriaxone Sodium during Pregnancy.Journal of microbiology and biotechnology · 2022Article
- Article
- The why and how of sleep-dependent synaptic down-selection.Seminars in cell & developmental biology · 2022Article
- Disrupted neural correlates of anesthesia and sleep reveal early circuit dysfunctions in Alzheimer models.Cell reports · 2022Article
- Overview of the Neuroprotective Effects of the MAO-Inhibiting Antidepressant Phenelzine.Cellular and molecular neurobiology · 2022Review
- Microdialysis and microperfusion electrodes in neurologic disease monitoring.Fluids and barriers of the CNS · 2021Review
- Net decrease in spine-surface GluA1-containing AMPA receptors after post-learning sleep in the adult mouse cortex.Nature communications · 2021Article
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Authors and funding
10 authors.
Funding
Abstract
By identifying endogenous molecules in brain extracellular fluid metabolomics can provide insight into the regulatory mechanisms and functions of sleep. Here we studied how the cortical metabolome changes during sleep, sleep deprivation and spontaneous wakefulness. Mice were implanted with electrodes for chronic sleep/wake recording and with microdialysis probes targeting prefrontal and primary motor cortex. Metabolites were measured using ultra performance liquid chromatography-high resolution mass spectrometry. Sleep/wake changes in metabolites were evaluated using partial least squares discriminant analysis, linear mixed effects model analysis of variance, and machine-learning algorithms. More than 30 known metabolites were reliably detected in most samples. When used by a logistic regression classifier, the profile of these metabolites across sleep, spontaneous wake, and enforced wake was sufficient to assign mice to their correct experimental group (pair-wise) in 80-100% of cases. Eleven of these metabolites showed significantly higher levels in awake than in sleeping mice. Some changes extend previous findings (glutamate, homovanillic acid, lactate, pyruvate, tryptophan, uridine), while others are novel (D-gluconate, N-acetyl-beta-alanine, N-acetylglutamine, orotate, succinate/methylmalonate). The upregulation of the de novo pyrimidine pathway, gluconate shunt and aerobic glycolysis may reflect a wake-dependent need to promote the synthesis of many essential components, from nucleic acids to synaptic membranes.
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