ArticleScientific reports2018
Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. It is linked to trial NCT05592795 (Explore the Effects and Mechanisms of ERCP and EST on Biliary Microecology), which is not on this map. Cited by 30 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Explore the Effects and Mechanisms of ERCP and EST on Biliary Microecology
Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- A systematic review on omics data (metagenomics, metatranscriptomics, and metabolomics) in the role of microbiome in gallbladder disease.Frontiers in physiology · 2022Pooled it
- Review
- Biliary Microbiota in Health and Disease: Clinical Implications in Lithiasis, Infection, and Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2026Review
- Biliary tract microbes and common bile duct stones: current status and prospects.Frontiers in microbiology · 2026Review
- Article
- Article
- Preliminary study of biliary microbiota and identification of bacterial species associated with pigmented gallstone formation.Frontiers in cellular and infection microbiology · 2025Article
- Biliary Microbial Community and Metabolic Potential in Patients with Multiple Common Bile Duct Stones.Clinical and experimental gastroenterology · 2025Article
- Progress in the Study of Intratumoral Microorganisms in Hepatocellular Carcinoma.Journal of hepatocellular carcinoma · 2025Review
- Characterization of biliary and duodenal microbiota in patients with primary and recurrent choledocholithiasis.Health information science and systems · 2024Article
- Biliary microbiome and gallstones: A silent friendship.World journal of gastrointestinal surgery · 2024Article
- The role of bacteria in gallstone formation.Folia microbiologica · 2024Review
- Gallbladder microbial species and host bile acids biosynthesis linked to cholesterol gallstone comparing to pigment individuals.Frontiers in cellular and infection microbiology · 2024Article
- Microbial characteristics of bile in gallstone patients: a comprehensive analysis of 9,939 cases.Frontiers in microbiology · 2024Article
- Fungal infections and the fungal microbiome in hepatobiliary disorders.Journal of hepatology · 2023Review
- Gastrointestinal microbiome and cholelithiasis: Current status and perspectives.World journal of gastroenterology · 2023Review
- Insight into the mechanism of gallstone disease by proteomic and metaproteomic characterization of human bile.Frontiers in microbiology · 2023Article
- Characteristics and metabolic potential of biliary microbiota in patients with giant common bile duct stones.Frontiers in cellular and infection microbiology · 2023Article
- The Role of the Gallbladder, the Intestinal Barrier and the Gut Microbiota in the Development of Food Allergies and Other Disorders.International journal of molecular sciences · 2022Review
- The Intratumoral Bacterial Metataxonomic Signature of Hepatocellular Carcinoma.Microbiology spectrum · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is growing evidence for bacteria playing a role in the pathogenesis and formation of pigmented gallstones from humans. These studies mainly involved cultivation of gallstone-associated bacteria and 16S rRNA profiling, providing an indirect link between processes involved in gallstone formation by the bacteria in-situ. Here, we provide functional metagenomic evidence of a range of genes involved in bile stress response, biofilm formation, and anaerobic energy metabolism by Gram-negative Klebsiella in pigmented gallstones from a 76-year-old male patient. Klebsiella was also present in one cholesterol-type stone in a 30-year-old female patient who had additional cholesterol gallstones characterised by Gram-positive bacteria. Pigmented stones further revealed a predominance of genes involved in carbohydrate metabolism, whilst cholesterol stones indicated a profile dominanted by protein metabolism possibly reflecting known chemical differences between Gram-negative and Gram-positive biofilm matrices. Archaeal genes were not detected. Complementary carbon and hydrogen isotopic analyses of cholesterol within the patients' stones revealed homogeneity, suggesting a common diet or cholesterol biosynthesis pathway that has little influence on microbial composition. This pilot study provides a framework to study microbial processes that play a potential role in gallstone formation across markedly different types of stones and patient backgrounds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.