Evidence map›Paper›PMID 30043358›Full record

ArticleJournal of the American Society for Mass Spectrometry2018

Multivariate Analysis of MALDI Imaging Mass Spectrometry Data of Mixtures of Single Pollen Grains.

Franziska Lauer, Sabrina Diehn, Stephan Seifert, Janina Kneipp, Volker Sauerland, Cesar Barahona, Steffen Weidner

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In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Franziska LauerBundesanstalt für Materialforschung und-prüfung (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
Sabrina DiehnBundesanstalt für Materialforschung und-prüfung (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
Stephan SeifertBundesanstalt für Materialforschung und-prüfung (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
Janina KneippBundesanstalt für Materialforschung und-prüfung (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
Volker SauerlandBruker Daltonik GmbH, Fahrenheitstraße 4, 28359, Bremen, Germany.
Cesar BarahonaBruker Daltonik GmbH, Fahrenheitstraße 4, 28359, Bremen, Germany.
Steffen WeidnerBundesanstalt für Materialforschung und-prüfung (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany. steffen.weidner@bam.de.ORCID http://orcid.org/0000-0001-8111-2765

Funding

European Research Council 259432
6 · The paper itself

Abstract

Mixtures of pollen grains of three different species (Corylus avellana, Alnus cordata, and Pinus sylvestris) were investigated by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF imaging MS). The amount of pollen grains was reduced stepwise from > 10 to single pollen grains. For sample pretreatment, we modified a previously applied approach, where any additional extraction steps were omitted. Our results show that characteristic pollen MALDI mass spectra can be obtained from a single pollen grain, which is the prerequisite for a reliable pollen classification in practical applications. MALDI imaging of laterally resolved pollen grains provides additional information by reducing the complexity of the MS spectra of mixtures, where frequently peak discrimination is observed. Combined with multivariate statistical analyses, such as principal component analysis (PCA), our approach offers the chance for a fast and reliable identification of individual pollen grains by mass spectrometry. Graphical Abstract ᅟ.

Indexed as

Cluster AnalysisMultivariate AnalysisPollenPrincipal Component AnalysisSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationHierarchical cluster analysisMALDI imaging MSMultivariate statisticsPollen grainsPrincipal component analysis

Identifiers

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.