Antagonistic effects of Spirulina platensis on diazinon-induced hemato-biochemical alterations and oxidative stress in rats.

Spirulina platensis (SP) is a traditionally used microalga for a wide range of pharmacological activities, including amelioration of heavy metals and pesticides toxicity. This study evaluated the antioxidant and organoprotective effects of SP against diazinon (DZN)-induced subacute toxicity on the blood, heart, liver, and kidneys of male Wistar albino rats. Diazinon (20 mg/kg, subcutaneous) was administered to animals either alone or along with an oral pure SP powder at doses of 500 and 1000 mg/kg. Alterations in hematological and serum biochemical parameters, as well as oxidative stress markers in the hepatic, renal, and cardiac tissues were evaluated, using colorimetric spectrophotometric techniques. The obtained results revealed that in comparison to the control group, DZN-treated rats exhibited significantly lower (p < 0.05) red blood cells and platelets counts, hemoglobin and hematocrit values, and activities of serum acetylcholinesterase and tissue antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase). Meanwhile, biochemical analysis showed significantly higher (p < 0.05) white blood cells count, serum concentrations of tumor necrosis factor-α and cardiac [creatine kinase (CK) and CK-muscle/brain fraction], hepatic [transaminases and alkaline phosphatase], and renal [uric acid, urea and creatinine] injury markers, and tissue levels of malondialdehyde (a marker of lipid peroxidation) in the DZN-intoxicated group, compared to normal controls. Interestingly, the administration of SP significantly ameliorated the previous hemato-biochemical alterations and mitigated DZN-induced organ injuries and oxidative stress. In conclusion, the natural antioxidant microalga (SP) effectively alleviated the DZN-induced hematologic alterations and organ injuries, probably through its antioxidant and anti-inflammatory activities.