Evidence map›Paper›PMID 30043336›Full record

ArticleJournal of assisted reproduction and genetics2018

Xenotransplantation of pre-pubertal ovarian cortex and prevention of follicle depletion with anti-Müllerian hormone (AMH).

Laura Detti, Nicole M Fletcher, Ghassan M Saed, Trevor W Sweatman, Rebecca A Uhlmann, Alberto Pappo, Irene Peregrin-Alvarez

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
  2. Review
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  5. Review
  6. The Effect of AMH on Folliculogenesis.Reproductive sciences (Thousand Oaks, Calif.) · 2025
    Review
  7. Review
  8. Article
  9. Review
  10. Article
  11. Review
  12. Research progress on mechanism of follicle injury after ovarian tissue transplantation and protective strategies.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2024
    Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Laura DettiDepartment of Obstetrics and Gynecology, University of Tennessee Health Science Center, Memphis, TN, USA. ldetti@uthsc.edu.ORCID http://orcid.org/0000-0001-6868-7185
Nicole M FletcherThe C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI, USA.
Ghassan M SaedThe C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI, USA.
Trevor W SweatmanDepartment of Pharmacology, University of Tennessee Health Science Center, Memphis, TN, USA.
Rebecca A UhlmannThe C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI, USA.
Alberto PappoSt. Jude Children's Research Hospital, Memphis, TN, USA.
Irene Peregrin-AlvarezDepartment of Obstetrics and Gynecology, University of Tennessee Health Science Center, Memphis, TN, USA.

Funding

Health Science Center, University of Tennessee E07-3225-001
6 · The paper itself

Abstract

objectiveTo determine whether recombinant AMH (rAMH) could prevent post-transplant follicular depletion by acting on the stemness markers Oct-4, Sox2, and NANOG. MATERIALS AND

methodsThis was an experimental study where 12 ovariectomized nude mice were xenotransplanted with vitrified/warmed ovarian cortex obtained from a pre-pubertal girl and Alzet pumps delivering rAMH, or placebo (control), were inserted intra-abdominally. Previously vitrified/warmed ovarian cortex fragments were transplanted after 7 days and then harvested after 14 days from pump placement. We performed real-time RT-PCR analyses, ELISA for AMH, FSH, and estradiol, histologic measurement of ovarian follicles, and immunohistochemistry for Ki67 and TUNEL. The main outcome measures were serum levels and tissue expression of the parameters under investigation and follicle count.

resultsSerum AMH, FSH, and estradiol reflected post-ovariectomy profiles and were mildly influenced by rAMH administration. Ovarian cortex expression of AMH, AMH-R2, VEGF, GDF9, Oct-4, and Sox2 was lower in rAMH mice than in controls, while NANOG was upregulated. There was a non-significant decrease in primordial follicles after vitrification-warming, and xenotransplantation further decreased this number. There were lower cell replication and depressed apoptosis in the rAMH group.

conclusionsAdministration of recombinant AMH in the peri-transplant period did not protect the initial follicular depletion but decreased apoptosis and cellular activation and regulated stem cell markers' tissue expression. These results aid our understanding of the inhibitory effects of AMH on follicular development and show the benefit of administering exogenous AMH at the time of pre-pubertal ovarian cortex transplant to protect the follicles from pre-activation and premature depletion.

Indexed as

AnimalsAnti-Mullerian HormoneApoptosisEstradiolFemaleFollicle Stimulating HormoneGene Expression Regulation, DevelopmentalHeterograftsHumansMiceNanog Homeobox ProteinOctamer Transcription Factor-3Ovarian FollicleOvariectomyOvarySOXB1 Transcription FactorsAnti-Mullerian HormoneEstradiolFollicle Stimulating HormoneNanog Homeobox ProteinNanog protein, mouseOctamer Transcription Factor-3Sox2 protein, mouseSOXB1 Transcription FactorsOvarian cortexPre-pubertalRecombinant AMHStemnessVitrificationXenotransplant

Identifiers

PMID30043336
PMCPMC6150887

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.