ArticleEuropean biophysics journal : EBJ2019
Correlative iPALM and SEM resolves virus cavity and Gag lattice defects in HIV virions.
Article in European biophysics journal : EBJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts.BBA advances · 2023Article
- Coexistence of vitreous and crystalline phases of HProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Metal-Induced Fluorescence Quenching of Photoconvertible Fluorescent Protein DendFP.Molecules (Basel, Switzerland) · 2022Article
- Gag-Gag Interactions Are Insufficient to Fully Stabilize and Order the Immature HIV Gag Lattice.Viruses · 2021Article
- Towards a Quantitative Single Particle Characterization by Super Resolution Microscopy: From Virus Structures to Antivirals Design.Frontiers in bioengineering and biotechnology · 2021Review
- Dynamics of the HIV Gag Lattice Detected by Localization Correlation Analysis and Time-Lapse iPALM.Biophysical journal · 2020Article
- Quality Assessment of Virus-Like Particles at Single Particle Level: A Comparative Study.Viruses · 2020Article
- High-speed imaging of ESCRT recruitment and dynamics during HIV virus like particle budding.PloS one · 2020Article
- Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy.Viruses · 2019Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Interferometric Photo-Activation-Localization-Microscopy (iPALM) localizes single fluorescent molecules with 20 nm lateral and 10 nm axial resolution. We present a method utilizing glass coverslip lithography for correlative imaging between iPALM and scanning electron microscopy (SEM). Using iPALM on HIV Gag-Dendra virus-like particles (VLPs) we localized the position of HIV Gag proteins. Based on these localizations we reconstructed the central cavity of the VLPs along with imperfections within the HIV Gag lattice. The SEM images and iPALM images overlap and show imaging from single VLPs immobilized on glass coverslips. The localization of many HIV proteins including accessory proteins and Gag-Pol remains unknown, we discuss how the specificity of iPALM coupled with SEM has the potential for resolving more of HIV proteins.
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