Evidence map›Paper›PMID 30042489›Full record

ArticleScientific reports2018

Durable targeting of B-lymphocytes in living mice.

M Cascalho, D Huynh, A R Lefferts, L Stein, T Lanigan, J Decker, L D Shea, J L Platt

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

M CascalhoDepartment of Microbiology & Immunology, University of Michigan, 1150 W, Medical Center Dr., Ann Arbor, MI, 48109-5656, USA. marilia@umich.edu.
D HuynhDepartment of Surgery, University of Michigan, 1150 W, Medical Center Dr., Ann Arbor, MI, 48109-5656, USA.
A R LeffertsDepartment of Surgery, University of Michigan, 1150 W, Medical Center Dr., Ann Arbor, MI, 48109-5656, USA.
L SteinDepartment of Surgery, University of Michigan, 1150 W, Medical Center Dr., Ann Arbor, MI, 48109-5656, USA.
T LaniganDepartment of Medicine, University of Michigan, 1150 W, Medical Center Dr., Ann Arbor, MI, 48109-5656, USA.
J DeckerDepartment of Bioengineering, University of Michigan, 1107 carl A. Gerstacker Building, 2200 Bonisteel Blv., Ann Arbor, MI, 48109-2099, USA.ORCID 0000-0001-7914-4741
L D SheaDepartment of Bioengineering, University of Michigan, 1107 carl A. Gerstacker Building, 2200 Bonisteel Blv., Ann Arbor, MI, 48109-2099, USA.
J L PlattDepartment of Microbiology & Immunology, University of Michigan, 1150 W, Medical Center Dr., Ann Arbor, MI, 48109-5656, USA. plattjl@umich.edu.
University of Michigan · US

Funding

Enhancing resistance to enteric pathogensR21AI117561 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CASCALHO, MARILIA ISABEL, PLATT, JEFFREY L · 2015 to 2016
$426k
Accommodation in TransplantationR56AI123262 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CASCALHO, MARILIA ISABEL, PLATT, JEFFREY L · 2016 to 2016
$388k
NIAID NIH HHS R21 AI117561NIAID NIH HHS R56 AI123262
6 · The paper itself

Abstract

Transfer to and enduring expression of genes in B cells has proved a vexing challenge. We report here a novel method for the specific and durable targeting of B lymphocytes in living mice. The method involves generation of lentiviruses pseudotyped with an anti-CD19 antibody. CD19 targeting viruses injected in the spleen of living mice efficiently transduced B cells and plasma cells detected by flow cytometry analysis of GFP expression. Expression of the reporter gene could be detected in the intact animal by external imaging for more than a year and was enhanced by booster immunization. Our method thus enables the specific delivery, expression and localization by external imaging of exogenous genes to the B cells and plasma cells of living individuals.

Indexed as

AnimalsAntibodies, Monoclonal, Murine-DerivedAntigens, CD19B-LymphocytesCell DifferentiationCell ProliferationFemaleFlow CytometryGenes, ReporterGenetic VectorsLentivirusLuciferasesLuminescent AgentsMaleMiceMice, Inbred C57BLAntibodies, Monoclonal, Murine-DerivedAntigens, CD19CD19 antigen, mouseLuciferasesLuminescent Agents

Identifiers

PMID30042489
PMCPMC6057982
OpenAlexW2883622287

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.